TY - JOUR
T1 - γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells
AU - Gallily, Ruth
AU - Even-Chen, Tal
AU - Katzavian, Galia
AU - Lehmann, Dan
AU - Dagan, Arie
AU - Mechoulam, Raphael
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
AB - Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.
KW - Acute myeloid leukemia
KW - Anandamide
KW - Apoptosis
KW - Cannabidiol
KW - Caspase
KW - HL-60
UR - http://www.scopus.com/inward/record.url?scp=0141758489&partnerID=8YFLogxK
U2 - 10.1080/1042819031000103917
DO - 10.1080/1042819031000103917
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 14692532
AN - SCOPUS:0141758489
SN - 1042-8194
VL - 44
SP - 1767
EP - 1773
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 10
ER -