γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

Ruth Gallily*, Tal Even-Chen, Galia Katzavian, Dan Lehmann, Arie Dagan, Raphael Mechoulam

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.

Original languageEnglish
Pages (from-to)1767-1773
Number of pages7
JournalLeukemia and Lymphoma
Volume44
Issue number10
DOIs
StatePublished - 1 Oct 2003

Keywords

  • Acute myeloid leukemia
  • Anandamide
  • Apoptosis
  • Cannabidiol
  • Caspase
  • HL-60

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