γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

Ruth Gallily; Tal Even-Chen; Galia Katzavian; Dan Lehmann; Arie Dagan; Raphael Mechoulam

doi:10.1080/1042819031000103917

γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

Ruth Gallily^*
, Tal Even-Chen
, Galia Katzavian
, Dan Lehmann
, Arie Dagan
, Raphael Mechoulam

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.

Original language	English
Pages (from-to)	1767-1773
Number of pages	7
Journal	Leukemia and Lymphoma
Volume	44
Issue number	10
DOIs	https://doi.org/10.1080/1042819031000103917
State	Published - 1 Oct 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acute myeloid leukemia
Anandamide
Apoptosis
Cannabidiol
Caspase
HL-60

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10.1080/1042819031000103917

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title = "γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells",

abstract = "Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43\% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95\%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.",

keywords = "Acute myeloid leukemia, Anandamide, Apoptosis, Cannabidiol, Caspase, HL-60",

author = "Ruth Gallily and Tal Even-Chen and Galia Katzavian and Dan Lehmann and Arie Dagan and Raphael Mechoulam",

year = "2003",

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T1 - γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

AU - Gallily, Ruth

AU - Even-Chen, Tal

AU - Katzavian, Galia

AU - Lehmann, Dan

AU - Dagan, Arie

AU - Mechoulam, Raphael

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.

AB - Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 μg/ml CBD and 15 μg/ml CBD-DMH, respectively, after a 24h treatment. Prior exposure of the cells to γ-irradiation (800cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or γ-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.

KW - Acute myeloid leukemia

KW - Anandamide

KW - Apoptosis

KW - Cannabidiol

KW - Caspase

KW - HL-60

UR - https://www.scopus.com/pages/publications/0141758489

U2 - 10.1080/1042819031000103917

DO - 10.1080/1042819031000103917

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AN - SCOPUS:0141758489

SN - 1042-8194

VL - 44

SP - 1767

EP - 1773

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

IS - 10

ER -

γ-Irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells

Abstract

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Keywords

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