TY - JOUR
T1 - 2.8-Å Cryo-EM Structure of the Large Ribosomal Subunit from the Eukaryotic Parasite Leishmania
AU - Shalev-Benami, Moran
AU - Zhang, Yan
AU - Matzov, Donna
AU - Halfon, Yehuda
AU - Zackay, Arie
AU - Rozenberg, Haim
AU - Zimmerman, Ella
AU - Bashan, Anat
AU - Jaffe, Charles L.
AU - Yonath, Ada
AU - Skiniotis, Georgios
N1 - Publisher Copyright:
© 2016
PY - 2016/7/12
Y1 - 2016/7/12
N2 - Leishmania is a single-cell eukaryotic parasite of the Trypanosomatidae family, whose members cause an array of tropical diseases. The often fatal outcome of infections, lack of effective vaccines, limited selection of therapeutic drugs, and emerging resistant strains, underline the need to develop strategies to combat these pathogens. The Trypanosomatid ribosome has recently been highlighted as a promising therapeutic target due to structural features that are distinct from other eukaryotes. Here, we present the 2.8-Å resolution structure of the Leishmania donovani large ribosomal subunit (LSU) derived from a cryo-EM map, further enabling the structural observation of eukaryotic rRNA modifications that play a significant role in ribosome assembly and function. The structure illustrates the unique fragmented nature of leishmanial LSU rRNA and highlights the irregular distribution of rRNA modifications in Leishmania, a characteristic with implications for anti-parasitic drug development.
AB - Leishmania is a single-cell eukaryotic parasite of the Trypanosomatidae family, whose members cause an array of tropical diseases. The often fatal outcome of infections, lack of effective vaccines, limited selection of therapeutic drugs, and emerging resistant strains, underline the need to develop strategies to combat these pathogens. The Trypanosomatid ribosome has recently been highlighted as a promising therapeutic target due to structural features that are distinct from other eukaryotes. Here, we present the 2.8-Å resolution structure of the Leishmania donovani large ribosomal subunit (LSU) derived from a cryo-EM map, further enabling the structural observation of eukaryotic rRNA modifications that play a significant role in ribosome assembly and function. The structure illustrates the unique fragmented nature of leishmanial LSU rRNA and highlights the irregular distribution of rRNA modifications in Leishmania, a characteristic with implications for anti-parasitic drug development.
UR - http://www.scopus.com/inward/record.url?scp=84978511982&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2016.06.014
DO - 10.1016/j.celrep.2016.06.014
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C2 - 27373148
AN - SCOPUS:84978511982
SN - 2211-1247
VL - 16
SP - 288
EP - 294
JO - Cell Reports
JF - Cell Reports
IS - 2
ER -