[78] Kinetic Resonance Raman Spectroscopy with Microsampling Rotating Cells

Aaron Lewis

Research output: Contribution to journalArticlepeer-review

Abstract

One of the technical achievements of resonance Raman spectroscopy of visual pigments and bacteriorhodopsin has been the introduction of a whole variety of kinetic techniques to the field of Raman spectroscopy. This focuses on one of these techniques: the use of rotating cells. The principles that apply to this technique are identical to all aspects of kinetic resonance Raman spectroscopy as they have been applied to rhodopsin and bacteriorhodopsin. The residence time of the sample in the laser beam is the kinetic parameter that is the basis of all kinetic resonance Raman measurements on photolabile retinylidene proteins. The transit time of the molecules in the laser beam determines whether there is sufficient time to produce a particular intermediate state, and determines thecomplex admixture of pigment states present in the laser beam.

Original languageEnglish
Pages (from-to)659-666
Number of pages8
JournalMethods in Enzymology
Volume88
Issue numberC
DOIs
StatePublished - 1 Jan 1982

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