A BAC-based physical map of the major autosomes of Drosophila melanogaster

Roger A. Hoskins; Catherine R. Nelson; Benjamin P. Berman; Todd R. Laverty; Reed A. George; Lisa Ciesiolka; Mohammed Naeemuddin; Andrew D. Arenson; James Durbin; Robert G. David; Paul E. Tabor; Michael R. Bailey; Denise R. DeShazo; Joseph Catanese; Aaron Mammoser; Kazutoyo Osoegawa; Pieter J. De Jong; Susan E. Celniker; Richard A. Gibbs; Gerald M. Rubin; Steven E. Scherer

doi:10.1126/science.287.5461.2271

A BAC-based physical map of the major autosomes of Drosophila melanogaster

Roger A. Hoskins^*, Catherine R. Nelson, Benjamin P. Berman, Todd R. Laverty, Reed A. George, Lisa Ciesiolka, Mohammed Naeemuddin, Andrew D. Arenson, James Durbin, Robert G. David, Paul E. Tabor, Michael R. Bailey, Denise R. DeShazo, Joseph Catanese, Aaron Mammoser, Kazutoyo Osoegawa, Pieter J. De Jong, Susan E. Celniker, Richard A. Gibbs, Gerald M. RubinSteven E. Scherer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

115 Scopus citations

Abstract

We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

Original language	English
Pages (from-to)	2271-2274
Number of pages	4
Journal	Science
Volume	287
Issue number	5461
DOIs	https://doi.org/10.1126/science.287.5461.2271
State	Published - 24 Mar 2000
Externally published	Yes

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Hoskins, R. A., Nelson, C. R., Berman, B. P., Laverty, T. R., George, R. A., Ciesiolka, L., Naeemuddin, M., Arenson, A. D., Durbin, J., David, R. G., Tabor, P. E., Bailey, M. R., DeShazo, D. R., Catanese, J., Mammoser, A., Osoegawa, K., De Jong, P. J., Celniker, S. E., Gibbs, R. A., ... Scherer, S. E. (2000). A BAC-based physical map of the major autosomes of Drosophila melanogaster. Science, 287(5461), 2271-2274. https://doi.org/10.1126/science.287.5461.2271

@article{94a77b12e22049beae98825225539923,

title = "A BAC-based physical map of the major autosomes of Drosophila melanogaster",

abstract = "We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.",

author = "Hoskins, {Roger A.} and Nelson, {Catherine R.} and Berman, {Benjamin P.} and Laverty, {Todd R.} and George, {Reed A.} and Lisa Ciesiolka and Mohammed Naeemuddin and Arenson, {Andrew D.} and James Durbin and David, {Robert G.} and Tabor, {Paul E.} and Bailey, {Michael R.} and DeShazo, {Denise R.} and Joseph Catanese and Aaron Mammoser and Kazutoyo Osoegawa and {De Jong}, {Pieter J.} and Celniker, {Susan E.} and Gibbs, {Richard A.} and Rubin, {Gerald M.} and Scherer, {Steven E.}",

year = "2000",

month = mar,

day = "24",

doi = "10.1126/science.287.5461.2271",

language = "אנגלית",

volume = "287",

pages = "2271--2274",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

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Hoskins, RA, Nelson, CR, Berman, BP, Laverty, TR, George, RA, Ciesiolka, L, Naeemuddin, M, Arenson, AD, Durbin, J, David, RG, Tabor, PE, Bailey, MR, DeShazo, DR, Catanese, J, Mammoser, A, Osoegawa, K, De Jong, PJ, Celniker, SE, Gibbs, RA, Rubin, GM & Scherer, SE 2000, 'A BAC-based physical map of the major autosomes of Drosophila melanogaster', Science, vol. 287, no. 5461, pp. 2271-2274. https://doi.org/10.1126/science.287.5461.2271

TY - JOUR

T1 - A BAC-based physical map of the major autosomes of Drosophila melanogaster

AU - Hoskins, Roger A.

AU - Nelson, Catherine R.

AU - Berman, Benjamin P.

AU - Laverty, Todd R.

AU - George, Reed A.

AU - Ciesiolka, Lisa

AU - Naeemuddin, Mohammed

AU - Arenson, Andrew D.

AU - Durbin, James

AU - David, Robert G.

AU - Tabor, Paul E.

AU - Bailey, Michael R.

AU - DeShazo, Denise R.

AU - Catanese, Joseph

AU - Mammoser, Aaron

AU - Osoegawa, Kazutoyo

AU - De Jong, Pieter J.

AU - Celniker, Susan E.

AU - Gibbs, Richard A.

AU - Rubin, Gerald M.

AU - Scherer, Steven E.

PY - 2000/3/24

Y1 - 2000/3/24

N2 - We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

AB - We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

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VL - 287

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JO - Science

JF - Science

IS - 5461

ER -

A BAC-based physical map of the major autosomes of Drosophila melanogaster

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