A BAC-based physical map of the major autosomes of Drosophila melanogaster

Roger A. Hoskins*, Catherine R. Nelson, Benjamin P. Berman, Todd R. Laverty, Reed A. George, Lisa Ciesiolka, Mohammed Naeemuddin, Andrew D. Arenson, James Durbin, Robert G. David, Paul E. Tabor, Michael R. Bailey, Denise R. DeShazo, Joseph Catanese, Aaron Mammoser, Kazutoyo Osoegawa, Pieter J. De Jong, Susan E. Celniker, Richard A. Gibbs, Gerald M. RubinSteven E. Scherer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

Original languageAmerican English
Pages (from-to)2271-2274
Number of pages4
JournalScience
Volume287
Issue number5461
DOIs
StatePublished - 24 Mar 2000
Externally publishedYes

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