A BAC-based physical map of the major autosomes of Drosophila melanogaster

Roger A. Hoskins; Catherine R. Nelson; Benjamin P. Berman; Todd R. Laverty; Reed A. George; Lisa Ciesiolka; Mohammed Naeemuddin; Andrew D. Arenson; James Durbin; Robert G. David; Paul E. Tabor; Michael R. Bailey; Denise R. DeShazo; Joseph Catanese; Aaron Mammoser; Kazutoyo Osoegawa; Pieter J. De Jong; Susan E. Celniker; Richard A. Gibbs; Gerald M. Rubin; Steven E. Scherer

doi:10.1126/science.287.5461.2271

A BAC-based physical map of the major autosomes of Drosophila melanogaster

Roger A. Hoskins^*
, Catherine R. Nelson
, Benjamin P. Berman
, Todd R. Laverty
, Reed A. George
, Lisa Ciesiolka
, Mohammed Naeemuddin
, Andrew D. Arenson
, James Durbin
, Robert G. David
, Paul E. Tabor
, Michael R. Bailey
, Denise R. DeShazo
, Joseph Catanese
, Aaron Mammoser
, Kazutoyo Osoegawa
, Pieter J. De Jong
, Susan E. Celniker
, Richard A. Gibbs
, Gerald M. Rubin

Steven E. Scherer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

Original language	English
Pages (from-to)	2271-2274
Number of pages	4
Journal	Science
Volume	287
Issue number	5461
DOIs	https://doi.org/10.1126/science.287.5461.2271
State	Published - 24 Mar 2000
Externally published	Yes

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Hoskins, R. A., Nelson, C. R., Berman, B. P., Laverty, T. R., George, R. A., Ciesiolka, L., Naeemuddin, M., Arenson, A. D., Durbin, J., David, R. G., Tabor, P. E., Bailey, M. R., DeShazo, D. R., Catanese, J., Mammoser, A., Osoegawa, K., De Jong, P. J., Celniker, S. E., Gibbs, R. A., ... Scherer, S. E. (2000). A BAC-based physical map of the major autosomes of Drosophila melanogaster. Science, 287(5461), 2271-2274. https://doi.org/10.1126/science.287.5461.2271

@article{94a77b12e22049beae98825225539923,

title = "A BAC-based physical map of the major autosomes of Drosophila melanogaster",

abstract = "We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81\% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.",

author = "Hoskins, \{Roger A.\} and Nelson, \{Catherine R.\} and Berman, \{Benjamin P.\} and Laverty, \{Todd R.\} and George, \{Reed A.\} and Lisa Ciesiolka and Mohammed Naeemuddin and Arenson, \{Andrew D.\} and James Durbin and David, \{Robert G.\} and Tabor, \{Paul E.\} and Bailey, \{Michael R.\} and DeShazo, \{Denise R.\} and Joseph Catanese and Aaron Mammoser and Kazutoyo Osoegawa and \{De Jong\}, \{Pieter J.\} and Celniker, \{Susan E.\} and Gibbs, \{Richard A.\} and Rubin, \{Gerald M.\} and Scherer, \{Steven E.\}",

year = "2000",

month = mar,

day = "24",

doi = "10.1126/science.287.5461.2271",

language = "אנגלית",

volume = "287",

pages = "2271--2274",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5461",

}

Hoskins, RA, Nelson, CR, Berman, BP, Laverty, TR, George, RA, Ciesiolka, L, Naeemuddin, M, Arenson, AD, Durbin, J, David, RG, Tabor, PE, Bailey, MR, DeShazo, DR, Catanese, J, Mammoser, A, Osoegawa, K, De Jong, PJ, Celniker, SE, Gibbs, RA, Rubin, GM & Scherer, SE 2000, 'A BAC-based physical map of the major autosomes of Drosophila melanogaster', Science, vol. 287, no. 5461, pp. 2271-2274. https://doi.org/10.1126/science.287.5461.2271

TY - JOUR

T1 - A BAC-based physical map of the major autosomes of Drosophila melanogaster

AU - Hoskins, Roger A.

AU - Nelson, Catherine R.

AU - Berman, Benjamin P.

AU - Laverty, Todd R.

AU - George, Reed A.

AU - Ciesiolka, Lisa

AU - Naeemuddin, Mohammed

AU - Arenson, Andrew D.

AU - Durbin, James

AU - David, Robert G.

AU - Tabor, Paul E.

AU - Bailey, Michael R.

AU - DeShazo, Denise R.

AU - Catanese, Joseph

AU - Mammoser, Aaron

AU - Osoegawa, Kazutoyo

AU - De Jong, Pieter J.

AU - Celniker, Susan E.

AU - Gibbs, Richard A.

AU - Rubin, Gerald M.

AU - Scherer, Steven E.

PY - 2000/3/24

Y1 - 2000/3/24

N2 - We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

AB - We constructed a bacterial artificial chromosome (BAC)- based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

UR - https://www.scopus.com/pages/publications/0034708791

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C2 - 10731150

AN - SCOPUS:0034708791

SN - 0036-8075

VL - 287

SP - 2271

EP - 2274

JO - Science

JF - Science

IS - 5461

ER -

A BAC-based physical map of the major autosomes of Drosophila melanogaster

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