A BAC/BIBAC-based physical map of chickpea, Cicer arietinum L

Xiaojun Zhang, Chantel F. Scheuring, Meiping Zhang, Jennifer J. Dong, Yang Zhang, James J. Huang, Mi Kyung Lee, Shahal Abbo, Amir Sherman, Dani Shtienberg, Weidong Chen, Fred Muehlbauer, Hong Bin Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Background: Chickpea (Cicer arietinum L.) is the third most important pulse crop worldwide. Despite its importance, relatively little is known about its genome. The availability of a genome-wide physical map allows rapid fine mapping of QTL, development of high-density genome maps, and sequencing of the entire genome. However, no such a physical map has been developed in chickpea.Results: We present a genome-wide, BAC/BIBAC-based physical map of chickpea developed by fingerprint analysis. Four chickpea BAC and BIBAC libraries, two of which were constructed in this study, were used. A total of 67,584 clones were fingerprinted, and 64,211 (~11.7 ×) of the fingerprints validated and used in the physical map assembly. The physical map consists of 1,945 BAC/BIBAC contigs, with each containing an average of 28.3 clones and having an average physical length of 559 kb. The contigs collectively span approximately 1,088 Mb. By using the physical map, we identified the BAC/BIBAC contigs containing or closely linked to QTL4.1 for resistance to Didymella rabiei (RDR) and QTL8 for days to first flower (DTF), thus further verifying the physical map and confirming its utility in fine mapping and cloning of QTL.Conclusion: The physical map represents the first genome-wide, BAC/BIBAC-based physical map of chickpea. This map, along with other genomic resources previously developed in the species and the genome sequences of related species (soybean, Medicago and Lotus), will provide a foundation necessary for many areas of advanced genomics research in chickpea and other legume species. The inclusion of transformation-ready BIBACs in the map greatly facilitates its utility in functional analysis of the legume genomes.

Original languageAmerican English
Article number501
JournalBMC Genomics
Issue number1
StatePublished - 17 Sep 2010

Bibliographical note

Funding Information:
Authors thank Yen-Hsuan Wu and Yun-Hua Liu for their kind assistance; and Guenter Kahl, Peter Winter, Paul Taylor and David A. Hoisington for their kind support for the project development. This research was supported by Research Grant Award No.: US-3870-60C from BARD, the United States-Israel Binational Agricultural Research and Development Fund.


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