A bacterial biosensor encoding a genetically modified LuxR receptor exhibits improved detection of Pseudomonas aeruginosa's biomarker molecule 2-aminoacetophenone

I. Kviatkovski, T. Yarnitzky, S. Shushan, O. Schwartz-Harari, R. Nir-Paz, Y. Helman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.

Original languageAmerican English
Pages (from-to)9218-9221
Number of pages4
JournalChemical Communications
Volume54
Issue number66
DOIs
StatePublished - 2018

Bibliographical note

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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