TY - JOUR
T1 - A basic trypsin- and chymotrypsin-inhibitor from groundnuts (Arachis hypogaea)
AU - Tur-Sinai, Ariela
AU - Birk, Yehudith
AU - Gertler, A.
AU - Rigbi, M.
PY - 1972/5/18
Y1 - 1972/5/18
N2 - A trypsin- and chymotrypsin-inhibitor has been isolated by extraction of defatted groundnut meal at pH 5, by ammonium sulfate precipitation, and successive column chromatography on DEAE-cellulose, calcium phosphate, and CM-cellulose. The inhibitor was found to be homogeneous by ultracentrifugation, acrylamide gel electrophoresis, gel filtration on Sephadex G-50, and isoelectrofocusing in acrylamide gels. The pure inhibitor has an E1 cm1 % of 2.5 at 280 nm. Its isoelectric point lies between pH 8 and 9. Its molecular weight is about 7500. It is stable between pH 2 and 11 and stable in water when maintained at 100 °C for 15 min. The inhibitor forms stable complexes with trypsin and chymotrypsin at molar ratios of approximately 1:1. The phenomenon of temporary inhibition could not be observed. The complex of the inhibitor with either of the two enzymes fails to inhibit the other enzyme.
AB - A trypsin- and chymotrypsin-inhibitor has been isolated by extraction of defatted groundnut meal at pH 5, by ammonium sulfate precipitation, and successive column chromatography on DEAE-cellulose, calcium phosphate, and CM-cellulose. The inhibitor was found to be homogeneous by ultracentrifugation, acrylamide gel electrophoresis, gel filtration on Sephadex G-50, and isoelectrofocusing in acrylamide gels. The pure inhibitor has an E1 cm1 % of 2.5 at 280 nm. Its isoelectric point lies between pH 8 and 9. Its molecular weight is about 7500. It is stable between pH 2 and 11 and stable in water when maintained at 100 °C for 15 min. The inhibitor forms stable complexes with trypsin and chymotrypsin at molar ratios of approximately 1:1. The phenomenon of temporary inhibition could not be observed. The complex of the inhibitor with either of the two enzymes fails to inhibit the other enzyme.
UR - http://www.scopus.com/inward/record.url?scp=0015514823&partnerID=8YFLogxK
U2 - 10.1016/0005-2795(72)90048-7
DO - 10.1016/0005-2795(72)90048-7
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C2 - 5034214
AN - SCOPUS:0015514823
SN - 0005-2795
VL - 263
SP - 666
EP - 672
JO - BBA - Protein Structure
JF - BBA - Protein Structure
IS - 3
ER -