A biochip model of lymphocyte locomotion on confined chemokine tracks

A biochip model of lymphocyte locomotion on confined chemokine tracks is described. Micropatterned chemokine biochips were constructed with the help microscope glass coverslips coated with daisy molecular films. Chemically reactive primary amines were fully de-protected at the solution termini of daisy by shining UV light. Biochips were assembled on slides mounted on an automated stage of an inverted microscope. The slides were assembled within a hermetically sealed chamber apparatus 0.3 mm in height to avoid fluid convection. Human T lymphocytes freshly isolated from blood were perfused through the chamber. Upon entry to the chamber flow was stopped, allowing cells to randomly settle on the CXCL12-presenting biochip. Movies were recorded over 30-min periods in multiple fields of view using Softmorx 3.5 at four frames per minute using a 20x/0.95 NA differential interference contrast objective.