TY - JOUR
T1 - A carbapenem-resistant Klebsiella pneumoniae epidemic clone in Jerusalem
T2 - Sequence type 512 carrying a plasmid encoding aac(6')-Ib
AU - Warburg, Gabriela
AU - Hidalgo-Grass, Carlos
AU - Partridge, Sally R.
AU - Tolmasky, Marcelo E.
AU - Temper, Violeta
AU - Moses, Allon E.
AU - Block, Colin
AU - Strahilevitz, Jacob
N1 - Funding Information:
This work was supported in part by grant Morasha 1833/07 from the Israel Science Foundation to J. S.
PY - 2012/4
Y1 - 2012/4
N2 - Objectives: We characterized distinctive features of a hypertransmissible carbapenem-resistant Klebsiella pneumoniae (CRKP) clone that emerged at Hadassah Hospital, Ein-Kerem, Jerusalem, Israel, in 2006. Methods: Eleven CRKP isolated at Hadassah Hospital during 2005-09 were examined by antimicrobial susceptibility testing, PFGE and multilocus sequence typing (MLST). Plasmids were analysed by conjugation, restriction mapping, PCR and sequencing. Results: Divergence from the national epidemic sequence type (ST) ST258 to ST512 was observed early on. Carbapenem resistance was conferred by bla KPC-3 carried on a plasmid apparently closely related to pKpQIL, also from Israel. This clone also carried a 15 kb plasmid, designated pAAC154, that carries a Tn1331 derivative containing the aac(6')-Ib gene. pAAC154 does not carry a bla KPC gene, but is similar to pS15, a plasmid from New York that carries bla KPC-2. Conclusions: A single CRKP clone ST512 has spread efficiently in our region. In this clone, aac(6')-Ib, common in CRKP strains, is carried on a different plasmid from bla KPC-3.
AB - Objectives: We characterized distinctive features of a hypertransmissible carbapenem-resistant Klebsiella pneumoniae (CRKP) clone that emerged at Hadassah Hospital, Ein-Kerem, Jerusalem, Israel, in 2006. Methods: Eleven CRKP isolated at Hadassah Hospital during 2005-09 were examined by antimicrobial susceptibility testing, PFGE and multilocus sequence typing (MLST). Plasmids were analysed by conjugation, restriction mapping, PCR and sequencing. Results: Divergence from the national epidemic sequence type (ST) ST258 to ST512 was observed early on. Carbapenem resistance was conferred by bla KPC-3 carried on a plasmid apparently closely related to pKpQIL, also from Israel. This clone also carried a 15 kb plasmid, designated pAAC154, that carries a Tn1331 derivative containing the aac(6')-Ib gene. pAAC154 does not carry a bla KPC gene, but is similar to pS15, a plasmid from New York that carries bla KPC-2. Conclusions: A single CRKP clone ST512 has spread efficiently in our region. In this clone, aac(6')-Ib, common in CRKP strains, is carried on a different plasmid from bla KPC-3.
KW - Aminoglycoside-modifying enzymes
KW - Carbapenemases
KW - Enterobacteriaceae
UR - http://www.scopus.com/inward/record.url?scp=84858672399&partnerID=8YFLogxK
U2 - 10.1093/jac/dkr552
DO - 10.1093/jac/dkr552
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C2 - 22287232
AN - SCOPUS:84858672399
SN - 0305-7453
VL - 67
SP - 898
EP - 901
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 4
M1 - dkr552
ER -
