A cellular and spatial map of the choroid plexus across brain ventricles and ages

Neil Dani; Rebecca H. Herbst; Cristin McCabe; Gilad S. Green; Karol Kaiser; Joshua P. Head; Jin Cui; Frederick B. Shipley; Ahram Jang; Danielle Dionne; Lan Nguyen; Christopher Rodman; Samantha J. Riesenfeld; Jan Prochazka; Michaela Prochazkova; Radislav Sedlacek; Feng Zhang; Vitezslav Bryja; Orit Rozenblatt-Rosen; Naomi Habib; Aviv Regev; Maria K. Lehtinen

doi:10.1016/j.cell.2021.04.003

A cellular and spatial map of the choroid plexus across brain ventricles and ages

Neil Dani, Rebecca H. Herbst, Cristin McCabe, Gilad S. Green, Karol Kaiser, Joshua P. Head, Jin Cui, Frederick B. Shipley, Ahram Jang, Danielle Dionne, Lan Nguyen, Christopher Rodman, Samantha J. Riesenfeld, Jan Prochazka, Michaela Prochazkova, Radislav Sedlacek, Feng Zhang, Vitezslav Bryja, Orit Rozenblatt-Rosen, Naomi Habib^*Aviv Regev^*, Maria K. Lehtinen^*

^*Corresponding author for this work

Edmond & Lily Safra Center for Brain Sciences

Research output: Contribution to journal › Article › peer-review

145 Scopus citations

Abstract

The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.

Original language	English
Pages (from-to)	3056-3074.e21
Journal	Cell
Volume	184
Issue number	11
DOIs	https://doi.org/10.1016/j.cell.2021.04.003
State	Published - 27 May 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

aging
brain barrier
cerebrospinal fluid
choroid plexus
development
single-cell RNA sequencing
single-nucleus RNA sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cell.2021.04.003

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Dani, N., Herbst, R. H., McCabe, C., Green, G. S., Kaiser, K., Head, J. P., Cui, J., Shipley, F. B., Jang, A., Dionne, D., Nguyen, L., Rodman, C., Riesenfeld, S. J., Prochazka, J., Prochazkova, M., Sedlacek, R., Zhang, F., Bryja, V., Rozenblatt-Rosen, O., ... Lehtinen, M. K. (2021). A cellular and spatial map of the choroid plexus across brain ventricles and ages. Cell, 184(11), 3056-3074.e21. https://doi.org/10.1016/j.cell.2021.04.003

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title = "A cellular and spatial map of the choroid plexus across brain ventricles and ages",

abstract = "The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.",

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author = "Neil Dani and Herbst, {Rebecca H.} and Cristin McCabe and Green, {Gilad S.} and Karol Kaiser and Head, {Joshua P.} and Jin Cui and Shipley, {Frederick B.} and Ahram Jang and Danielle Dionne and Lan Nguyen and Christopher Rodman and Riesenfeld, {Samantha J.} and Jan Prochazka and Michaela Prochazkova and Radislav Sedlacek and Feng Zhang and Vitezslav Bryja and Orit Rozenblatt-Rosen and Naomi Habib and Aviv Regev and Lehtinen, {Maria K.}",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

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day = "27",

doi = "10.1016/j.cell.2021.04.003",

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Dani, N, Herbst, RH, McCabe, C, Green, GS, Kaiser, K, Head, JP, Cui, J, Shipley, FB, Jang, A, Dionne, D, Nguyen, L, Rodman, C, Riesenfeld, SJ, Prochazka, J, Prochazkova, M, Sedlacek, R, Zhang, F, Bryja, V, Rozenblatt-Rosen, O, Habib, N, Regev, A & Lehtinen, MK 2021, 'A cellular and spatial map of the choroid plexus across brain ventricles and ages', Cell, vol. 184, no. 11, pp. 3056-3074.e21. https://doi.org/10.1016/j.cell.2021.04.003

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T1 - A cellular and spatial map of the choroid plexus across brain ventricles and ages

AU - Dani, Neil

AU - Herbst, Rebecca H.

AU - McCabe, Cristin

AU - Green, Gilad S.

AU - Kaiser, Karol

AU - Head, Joshua P.

AU - Cui, Jin

AU - Shipley, Frederick B.

AU - Jang, Ahram

AU - Dionne, Danielle

AU - Nguyen, Lan

AU - Rodman, Christopher

AU - Riesenfeld, Samantha J.

AU - Prochazka, Jan

AU - Prochazkova, Michaela

AU - Sedlacek, Radislav

AU - Zhang, Feng

AU - Bryja, Vitezslav

AU - Rozenblatt-Rosen, Orit

AU - Habib, Naomi

AU - Regev, Aviv

AU - Lehtinen, Maria K.

PY - 2021/5/27

Y1 - 2021/5/27

N2 - The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.

AB - The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.

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KW - brain barrier

KW - cerebrospinal fluid

KW - choroid plexus

KW - development

KW - single-cell RNA sequencing

KW - single-nucleus RNA sequencing

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U2 - 10.1016/j.cell.2021.04.003

DO - 10.1016/j.cell.2021.04.003

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C2 - 33932339

AN - SCOPUS:85106858617

SN - 0092-8674

VL - 184

SP - 3056-3074.e21

JO - Cell

JF - Cell

IS - 11

ER -

A cellular and spatial map of the choroid plexus across brain ventricles and ages

Abstract

Bibliographical note

Keywords

UN SDGs

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