A cellular and spatial map of the choroid plexus across brain ventricles and ages

Neil Dani, Rebecca H. Herbst, Cristin McCabe, Gilad S. Green, Karol Kaiser, Joshua P. Head, Jin Cui, Frederick B. Shipley, Ahram Jang, Danielle Dionne, Lan Nguyen, Christopher Rodman, Samantha J. Riesenfeld, Jan Prochazka, Michaela Prochazkova, Radislav Sedlacek, Feng Zhang, Vitezslav Bryja, Orit Rozenblatt-Rosen, Naomi Habib*Aviv Regev*, Maria K. Lehtinen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

122 Scopus citations


The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1β (IL-1β) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.

Original languageAmerican English
Pages (from-to)3056-3074.e21
Issue number11
StatePublished - 27 May 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Inc.


  • aging
  • brain barrier
  • cerebrospinal fluid
  • choroid plexus
  • development
  • single-cell RNA sequencing
  • single-nucleus RNA sequencing


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