A chromosomal region of Mycoplasma agalactiae containing vsp-related genes undergoes in vivo rearrangement in naturally infected animals

Ravenna Flitman-Tene, Sharon Levisohn, Inessa Lysnyansky, Eitan Rapoport, David Yogev*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A Mycoplasma agalactiae genomic fragment carrying four vsp-related genes (designated avg: agalactiae variable genes) was cloned, sequenced and compared to the vspA gene of Mycoplasma bovis. The following features were revealed: (i) the presence of a highly conserved vsp 5' upstream region; (ii) a highly homologous vsp N-terminal end encoding a putative lipoprotein signal sequence; (iii) sequence divergence of the rest of the mature proteins. By using avg specific probes in Southern blot analysis of genomic DNAs of M. agalactiae strains as well as of isolates from infected animals, marked DNA polymorphism of avg fragments was demonstrated. In addition, the avg genomic fingerprints were monitored for a period of 7 months, in isolates of M. agalactiae from an individual chronically infected animal. The results provided evidence that a chromosomal region of M. agalactiae, carrying vsp-related genes, undergoes rearrangements in vivo in the natural animal host during the course of infection. Copyright (C) 2000 Federation of European Microbiological Societies.

Original languageAmerican English
Pages (from-to)205-212
Number of pages8
JournalFEMS Microbiology Letters
Volume191
Issue number2
DOIs
StatePublished - 15 Oct 2000

Bibliographical note

Funding Information:
The authors would like to express their appreciation to Dr. J.G. Tully (Frederick, MD, USA), Dr. G. Jones (Edinburgh, UK) and Dr. K. Sachse (Jena, Germany) for supplying specific antisera used for identification of M. agalactiae and M. bovis isolates. This work was supported by a Grant (IS-2540-95R) from the United States–Israel Binational Agricultural Research and Development Fund (BARD).

Keywords

  • Genomic rearrangement
  • Mycoplasma agalactiae
  • Mycoplasma bovis
  • avg
  • vsp

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