A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities

Tetsuo Hamamoto; Oded Shoseyov; Frances Foong; Roy H. Doi

doi:10.1016/0378-1097(90)90318-K

A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities

Tetsuo Hamamoto, Oded Shoseyov, Frances Foong, Roy H. Doi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β-d-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli. The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.

Original language	English
Pages (from-to)	285-288
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	72
Issue number	3
DOIs	https://doi.org/10.1016/0378-1097(90)90318-K
State	Published - Nov 1990
Externally published	Yes

Bibliographical note

Funding Information:
The research was supported in part by Department of Energy Grant DE-FG03-87ERI3705. O.S. was supported by Fellowship SI-0057-87 from the United States-Israel Binational Agricultural Research and Development Fund.

Keywords

Cellobiosidase
Clostridium cellulovorans
Endoglucanase
Molecular cloning

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10.1016/0378-1097(90)90318-K

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title = "A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities",

abstract = "A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β-d-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli. The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.",

keywords = "Cellobiosidase, Clostridium cellulovorans, Endoglucanase, Molecular cloning",

author = "Tetsuo Hamamoto and Oded Shoseyov and Frances Foong and Doi, {Roy H.}",

note = "Funding Information: The research was supported in part by Department of Energy Grant DE-FG03-87ERI3705. O.S. was supported by Fellowship SI-0057-87 from the United States-Israel Binational Agricultural Research and Development Fund.",

year = "1990",

month = nov,

doi = "10.1016/0378-1097(90)90318-K",

language = "אנגלית",

volume = "72",

pages = "285--288",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

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AU - Hamamoto, Tetsuo

AU - Shoseyov, Oded

AU - Foong, Frances

AU - Doi, Roy H.

N1 - Funding Information: The research was supported in part by Department of Energy Grant DE-FG03-87ERI3705. O.S. was supported by Fellowship SI-0057-87 from the United States-Israel Binational Agricultural Research and Development Fund.

PY - 1990/11

Y1 - 1990/11

N2 - A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β-d-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli. The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.

AB - A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β-d-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli. The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.

KW - Cellobiosidase

KW - Clostridium cellulovorans

KW - Endoglucanase

KW - Molecular cloning

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VL - 72

SP - 285

EP - 288

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities

Abstract

Bibliographical note

Keywords

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