A Clostridium cellulovorans gene, engD, codes for both endo-β-1,4-glucanase and cellobiosidase activities

Tetsuo Hamamoto, Oded Shoseyov, Frances Foong, Roy H. Doi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β-d-glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli. The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000.

Original languageAmerican English
Pages (from-to)285-288
Number of pages4
JournalFEMS Microbiology Letters
Volume72
Issue number3
DOIs
StatePublished - Nov 1990
Externally publishedYes

Bibliographical note

Funding Information:
The research was supported in part by Department of Energy Grant DE-FG03-87ERI3705. O.S. was supported by Fellowship SI-0057-87 from the United States-Israel Binational Agricultural Research and Development Fund.

Keywords

  • Cellobiosidase
  • Clostridium cellulovorans
  • Endoglucanase
  • Molecular cloning

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