A comparative genomic analysis of the calcium signaling machinery in Neurospora crassa, Magnaporthe grisea, and Saccharomyces cerevisiae

Alex Zelter, Mojca Bencina, Barry J. Bowman, Oded Yarden, Nick D. Read*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

A large number of Ca2+-signaling proteins have been previously identified and characterized in Saccharomyces cerevisiae but relatively few have been discovered in filamentous fungi. In this study, a detailed, comparative genomic analysis of Ca2+-signaling proteins in Neurospora crassa, Magnaporthe grisea, and S. cerevisiae has been made. Our BLAST analysis identified 48, 42, and 40 Ca2+-signaling proteins in N. crassa, M. grisea, and S. cerevisiae, respectively. In N. crassa, M. grisea, and S. cerevisiae, 79, 100, and 13% of these proteins, respectively, were previously unknown. For N. crassa, M. grisea, and S. cerevisiae, respectively, we have identified: three Ca2+-permeable channels in each species; 9, 12, and 5 Ca2+/cation-ATPases; eight, six, and four Ca2+- exchangers; four, four, and two phospholipase C's; one calmodulin in each species; and 23, 21, and 29 Ca2+/calmodulin-regulated proteins. Homologs of a number of key proteins involved in the release of Ca2+ from intracellular stores, and in the sensing of extracellular Ca2+, in animal and plant cells, were not identified. The greater complexity of the Ca2+-signaling machinery in N. crassa and M. grisea over that in S. cerevisiae probably reflects their more complex cellular organization and behavior, and the greater range of external signals which filamentous fungi have to respond to in their natural habitats. To complement the data presented in this paper, a comprehensive web-based database resource (http://www.fungalcell. org/fdf/) of all Ca2+-signaling proteins identified in N. crassa, M. grisea, and S. cerevisiae has been provided.

Original languageAmerican English
Pages (from-to)827-841
Number of pages15
JournalFungal Genetics and Biology
Volume41
Issue number9
DOIs
StatePublished - Sep 2004

Bibliographical note

Funding Information:
We thank the Wellcome Trust (Grant No. 066392/Z/01/Z), the Israel Science Foundation, the British Council and the Slovenian Ministry of Education, Science and Sport for funding. We are also grateful to the Whitehead Institute/MIT Center for Genome Research ( http://www-genome.wi.mit.edu ) for conducting the Neurospora and Magnaporthe Sequencing Projects.

Keywords

  • Budding yeast
  • Calcium ATPase
  • Calcium channel
  • Calcium signaling proteins
  • Calcium transporter
  • Genomic analysis
  • Magnaporthe grisea
  • Neurospora crassa
  • Saccharomyces cerevisiae
  • Signal transduction

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