A comparative study of Iron uptake rates and mechanisms amongst marine and fresh water Cyanobacteria: prevalence of reductive Iron uptake

In this contribution, we address the question of iron bioavailability to cyanobacteria by measuring Fe uptake rates and probing for a reductive uptake pathway in diverse cyanobacterial species. We examined three Fe-substrates: dissolved inorganic iron (Fe') and the Fe-siderophores Ferrioxamine B (FOB) and FeAerobactin (FeAB). In order to compare across substrates and strains, we extracted uptake rate constants (k_in = uptake rate/[Fe-substrate]). Fe' was the most bioavailable Fe form to cyanobacteria, with k_in values higher than those of other substrates. When accounting for surface area (SA), all strains acquired Fe' at similar rates, as their k_in/SA were similar. We also observed homogeneity in the uptake of FOB among strains, but with 10,000 times lower k_in/SA values than Fe'. Uniformity in k_in/SA suggests similarity in the mechanism of uptake and indeed, all strains were found to employ a reductive step in the uptake of Fe' and FOB. In contrast, different uptake pathways were found for FeAB along with variations in k_in/SA. Our data supports the existence of a common reductive Fe uptake pathway amongst cyanobacteria, functioning alone or in addition to siderophore-mediated uptake. Cyanobacteria combining both uptake strategies benefit from increased flexibility in accessing different Fe-substrates.