TY - JOUR
T1 - A comparison of the second harmonic generation from light-adapted, dark-adapted, blue, and acid purple membrane
AU - Chen, Z.
AU - Sheves, M.
AU - Lewis, A.
AU - Bouevitch, O.
PY - 1994
Y1 - 1994
N2 - The second order nonlinear polarizability and dipole moment changes upon light excitation of light-adapted bacteriorhodopsin (BR), dark-adapted BR, blue membrane, and acid purple membrane have been measured by second harmonic generation. Our results indicate that the dipole moment changes of the retinal chromophore, delta mu, are very sensitive to both the chromophore structure and protein/chromophore interactions. Delta mu of light-adapted BR is larger than that of dark-adapted BR. The acid-induced formation of the blue membrane results in an increase in the delta mu value, and formation of acid purple membrane, resulting from further reduction of pH to 0, returns the delta mu to that of light-adapted BR. The implications of these findings are discussed.
AB - The second order nonlinear polarizability and dipole moment changes upon light excitation of light-adapted bacteriorhodopsin (BR), dark-adapted BR, blue membrane, and acid purple membrane have been measured by second harmonic generation. Our results indicate that the dipole moment changes of the retinal chromophore, delta mu, are very sensitive to both the chromophore structure and protein/chromophore interactions. Delta mu of light-adapted BR is larger than that of dark-adapted BR. The acid-induced formation of the blue membrane results in an increase in the delta mu value, and formation of acid purple membrane, resulting from further reduction of pH to 0, returns the delta mu to that of light-adapted BR. The implications of these findings are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0027978932&partnerID=8YFLogxK
U2 - 10.1016/S0006-3495(94)80583-1
DO - 10.1016/S0006-3495(94)80583-1
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C2 - 7811928
AN - SCOPUS:0027978932
SN - 0006-3495
VL - 67
SP - 1155
EP - 1160
JO - Biophysical Journal
JF - Biophysical Journal
IS - 3
ER -