TY - JOUR
T1 - A convenient fluorometric method for the detection of extracellular N-acetylglucosaminidase production by filamentous fungi
AU - Omero, Carmi
AU - Horwitz, Benjamin A.
AU - Chet, Ilan
PY - 2001
Y1 - 2001
N2 - A rapid and convenient method for the detection of chitinases accumulating in filamentous fungal cultures was developed. The assay is performed on cultures growing in microtiter plates, with a fluorogenic substrate: 4-methylumbelliferyl-N-acetyl-D-glucosaminide (4-MeUNAG). The fluorescence of the product, 4-methylumbelliferone, was detected. This method was successfully used to follow induction and repression of extracellular exochitinase activity in the biocontrol fungus Trichoderma harzianum. (C) 2001 Elsevier Science B.V.
AB - A rapid and convenient method for the detection of chitinases accumulating in filamentous fungal cultures was developed. The assay is performed on cultures growing in microtiter plates, with a fluorogenic substrate: 4-methylumbelliferyl-N-acetyl-D-glucosaminide (4-MeUNAG). The fluorescence of the product, 4-methylumbelliferone, was detected. This method was successfully used to follow induction and repression of extracellular exochitinase activity in the biocontrol fungus Trichoderma harzianum. (C) 2001 Elsevier Science B.V.
KW - 4-Methylumb elliferone
KW - 4-Methylumbelliferyl-N-acetyl-D-glucosaminide
KW - Chitinases
KW - Filamentous fungal culture
KW - Trichoderma harzianum
UR - http://www.scopus.com/inward/record.url?scp=0035212132&partnerID=8YFLogxK
U2 - 10.1016/S0167-7012(00)00213-X
DO - 10.1016/S0167-7012(00)00213-X
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C2 - 11118651
AN - SCOPUS:0035212132
SN - 0167-7012
VL - 43
SP - 165
EP - 169
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 3
ER -