Abstract
A rapid and convenient method for the detection of chitinases accumulating in filamentous fungal cultures was developed. The assay is performed on cultures growing in microtiter plates, with a fluorogenic substrate: 4-methylumbelliferyl-N-acetyl-D-glucosaminide (4-MeUNAG). The fluorescence of the product, 4-methylumbelliferone, was detected. This method was successfully used to follow induction and repression of extracellular exochitinase activity in the biocontrol fungus Trichoderma harzianum. (C) 2001 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 165-169 |
| Number of pages | 5 |
| Journal | Journal of Microbiological Methods |
| Volume | 43 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2001 |
Keywords
- 4-Methylumb elliferone
- 4-Methylumbelliferyl-N-acetyl-D-glucosaminide
- Chitinases
- Filamentous fungal culture
- Trichoderma harzianum
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