A direct spectrophotometric assay and determination of Michaelis constants for the β-lactamase reaction

Amram Samuni*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

The previously applied direct spectrophotometric assay of β-lactamase activity toward cephalosporins was found readily applicable to penicillins too. The differential uv absorption spectra of various penicillins and their corresponding penicilloic acids were determined. The appropriate experimental conditions were examined and the spectrophotometric assay seems adequate for the study of several substrates in a mixture. Also this method was found highly suitable for computerized analysis of the kinetic data for the determination of Michaelis constants of the various penicillins. The use of the integrated form of the rate equation for the evaluation of the best estimates of Michaelis constants was found advantageous.

Original languageEnglish
Pages (from-to)17-26
Number of pages10
JournalAnalytical Biochemistry
Volume63
Issue number1
DOIs
StatePublished - Jan 1975

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