A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus

Vicky Dritsou; Pantelis Topalis; Nikolai Windbichler; Alekos Simoni; Ann Hall; Daniel Lawson; Malcolm Hinsley; Daniel Hughes; Valerio Napolioni; Francesca Crucianelli; Elena Deligianni; Giuliano Gasperi; Ludvik M. Gomulski; Grazia Savini; Mosè Manni; Francesca Scolari; Anna R. Malacrida; Bruno Arcà; Jose M. Ribeiro; Fabrizio Lombardo; Giuseppe Saccone; Marco Salvemini; Riccardo Moretti; Giuseppe Aprea; Maurizio Calvitti; Matteo Picciolini; Philippos Aris Papathanos; Roberta Spaccapelo; Guido Favia; Andrea Crisanti; Christos Louis

doi:10.1179/2047773215Y.0000000031

A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus

Vicky Dritsou, Pantelis Topalis, Nikolai Windbichler, Alekos Simoni, Ann Hall, Daniel Lawson, Malcolm Hinsley, Daniel Hughes, Valerio Napolioni, Francesca Crucianelli, Elena Deligianni, Giuliano Gasperi, Ludvik M. Gomulski, Grazia Savini, Mosè Manni, Francesca Scolari, Anna R. Malacrida, Bruno Arcà, Jose M. Ribeiro, Fabrizio LombardoGiuseppe Saccone, Marco Salvemini, Riccardo Moretti, Giuseppe Aprea, Maurizio Calvitti, Matteo Picciolini, Philippos Aris Papathanos, Roberta Spaccapelo, Guido Favia, Andrea Crisanti, Christos Louis^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The draft genome sequence of Italian specimens of the Asian tiger mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae) was determined using a standard NGS (next generation sequencing) approach. The size of the assembled genome is comparable to that of Aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive DNA is concerned, most of which is made up of transposable elements. Although, based on BUSCO (Benchmarking Universal Single-Copy Orthologues) analysis, the genome assembly reported here contains more than 99% of protein-coding genes, several of those are expected to be represented in the assembly in a fragmented state. We also present here the annotation of several families of genes (tRNA genes, miRNA genes, the sialome, genes involved in chromatin condensation, sex determination genes, odorant binding proteins and odorant receptors). These analyses confirm that the assembly can be used for the study of the biology of this invasive vector of disease.

Original language	English
Pages (from-to)	207-220
Number of pages	14
Journal	Pathogens and Global Health
Volume	109
Issue number	5
DOIs	https://doi.org/10.1179/2047773215Y.0000000031
State	Published - Jul 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© W. S. Maney & Son Ltd 2015.

Keywords

BUSCO
Chikungunya
Dengue fever
Disease vector
Invasive species
NGS
Repetitive DNA
Transposable elements
WGS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1179/2047773215Y.0000000031

Cite this

Dritsou, V., Topalis, P., Windbichler, N., Simoni, A., Hall, A., Lawson, D., Hinsley, M., Hughes, D., Napolioni, V., Crucianelli, F., Deligianni, E., Gasperi, G., Gomulski, L. M., Savini, G., Manni, M., Scolari, F., Malacrida, A. R., Arcà, B., Ribeiro, J. M., ... Louis, C. (2015). A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus. Pathogens and Global Health, 109(5), 207-220. https://doi.org/10.1179/2047773215Y.0000000031

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title = "A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus",

abstract = "The draft genome sequence of Italian specimens of the Asian tiger mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae) was determined using a standard NGS (next generation sequencing) approach. The size of the assembled genome is comparable to that of Aedes aegypti; the two mosquitoes are also similar as far as the high content of repetitive DNA is concerned, most of which is made up of transposable elements. Although, based on BUSCO (Benchmarking Universal Single-Copy Orthologues) analysis, the genome assembly reported here contains more than 99% of protein-coding genes, several of those are expected to be represented in the assembly in a fragmented state. We also present here the annotation of several families of genes (tRNA genes, miRNA genes, the sialome, genes involved in chromatin condensation, sex determination genes, odorant binding proteins and odorant receptors). These analyses confirm that the assembly can be used for the study of the biology of this invasive vector of disease.",

keywords = "BUSCO, Chikungunya, Dengue fever, Disease vector, Invasive species, NGS, Repetitive DNA, Transposable elements, WGS",

author = "Vicky Dritsou and Pantelis Topalis and Nikolai Windbichler and Alekos Simoni and Ann Hall and Daniel Lawson and Malcolm Hinsley and Daniel Hughes and Valerio Napolioni and Francesca Crucianelli and Elena Deligianni and Giuliano Gasperi and Gomulski, {Ludvik M.} and Grazia Savini and Mos{\`e} Manni and Francesca Scolari and Malacrida, {Anna R.} and Bruno Arc{\`a} and Ribeiro, {Jose M.} and Fabrizio Lombardo and Giuseppe Saccone and Marco Salvemini and Riccardo Moretti and Giuseppe Aprea and Maurizio Calvitti and Matteo Picciolini and Papathanos, {Philippos Aris} and Roberta Spaccapelo and Guido Favia and Andrea Crisanti and Christos Louis",

note = "Publisher Copyright: {\textcopyright} W. S. Maney & Son Ltd 2015.",

year = "2015",

month = jul,

doi = "10.1179/2047773215Y.0000000031",

language = "אנגלית",

volume = "109",

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Dritsou, V, Topalis, P, Windbichler, N, Simoni, A, Hall, A, Lawson, D, Hinsley, M, Hughes, D, Napolioni, V, Crucianelli, F, Deligianni, E, Gasperi, G, Gomulski, LM, Savini, G, Manni, M, Scolari, F, Malacrida, AR, Arcà, B, Ribeiro, JM, Lombardo, F, Saccone, G, Salvemini, M, Moretti, R, Aprea, G, Calvitti, M, Picciolini, M, Papathanos, PA, Spaccapelo, R, Favia, G, Crisanti, A & Louis, C 2015, 'A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus', Pathogens and Global Health, vol. 109, no. 5, pp. 207-220. https://doi.org/10.1179/2047773215Y.0000000031

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AU - Dritsou, Vicky

AU - Topalis, Pantelis

AU - Windbichler, Nikolai

AU - Simoni, Alekos

AU - Hall, Ann

AU - Lawson, Daniel

AU - Hinsley, Malcolm

AU - Hughes, Daniel

AU - Napolioni, Valerio

AU - Crucianelli, Francesca

AU - Deligianni, Elena

AU - Gasperi, Giuliano

AU - Gomulski, Ludvik M.

AU - Savini, Grazia

AU - Manni, Mosè

AU - Scolari, Francesca

AU - Malacrida, Anna R.

AU - Arcà, Bruno

AU - Ribeiro, Jose M.

AU - Lombardo, Fabrizio

AU - Saccone, Giuseppe

AU - Salvemini, Marco

AU - Moretti, Riccardo

AU - Aprea, Giuseppe

AU - Calvitti, Maurizio

AU - Picciolini, Matteo

AU - Papathanos, Philippos Aris

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AU - Favia, Guido

AU - Crisanti, Andrea

AU - Louis, Christos

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KW - BUSCO

KW - Chikungunya

KW - Dengue fever

KW - Disease vector

KW - Invasive species

KW - NGS

KW - Repetitive DNA

KW - Transposable elements

KW - WGS

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SN - 2047-7724

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JF - Pathogens and Global Health

IS - 5

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A draft genome sequence of an invasive mosquito: An Italian Aedes albopictus

Abstract

Bibliographical note

Keywords

UN SDGs

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