A feasibility study of multi-site,intracellular recordings from mammalian neurons by extracellular gold mushroom-shaped microelectrodes

Silviya M. Ojovan*, Noha Rabieh, Nava Shmoel, Hadas Erez, Eilon Maydan, Ariel Cohen, Micha E. Spira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The development of multi-electrode array platforms for large scale recording of neurons is at the forefront of neuro-engineering research efforts. Recently we demonstrated, at the proof-of-concept level, a breakthrough neuron-microelectrode interface in which cultured Aplysia neurons tightly engulf gold mushroom-shaped microelectrodes (gMμEs). While maintaining their extracellular position, the gMμEs record synaptic- and action-potentials with characteristic features of intracellular recordings. Here we examined the feasibility of using gMμEs for intracellular recordings from mammalian neurons. To that end we experimentally examined the innate size limits of cultured rat hippocampal neurons to engulf gMμEs and measured the width of the extracellular cleft formed between the neurons and the gold surface. Using the experimental results we next analyzed the expected range of gMμEs-neuron electrical coupling coefficients. We estimated that sufficient electrical coupling levels to record attenuated synaptic- and action-potentials can be reached using the gMμE-neuron configuration. The definition of the engulfment limits of the gMμEs caps diameter at ‰ 2-2.5 €‰μm and the estimated electrical coupling coefficients from the simulations pave the way for rational development and application of the gMμE based concept for in-cell recordings from mammalian neurons.

Original languageAmerican English
Article number14100
JournalScientific Reports
Volume5
DOIs
StatePublished - 14 Sep 2015

Bibliographical note

Funding Information:
This study was supported by the Charles E. Smith and Prof. Elkes Laboratory for Collaborative Research in Psychobiology at the Hebrew University of Jerusalem, EU FP7 MERIDIAN grant agreement No. 280778, EU FP7 Future Emerging Technology program, “BRAINLEAP” grant No. 306502 and EU FP7 Marie Curie ITG Grant agreement No. 264872.

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