A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Nicolò Accanto*, François G.C. Blot*, Antonio Lorca Camara, Valeria Zampini, Florence Bui, Christophe Tourain, Noam Badt, Ori Katz, Valentina Emiliani

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a new micro-endoscope based on a fiber bundle and a GRIN lens, capable of 2P holographic photostimulation of multiple neurons with single cell precision and fast (100 Hz) 2P calcium imaging on fields of view of 250 μm in freely moving mice.

Original languageAmerican English
Title of host publicationOptics and the Brain, BRAIN 2022
PublisherOptica Publishing Group (formerly OSA)
ISBN (Electronic)9781957171036
StatePublished - 2022
EventOptics and the Brain, BRAIN 2022 - Fort Lauderdale, United States
Duration: 24 Apr 202227 Apr 2022

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Conference

ConferenceOptics and the Brain, BRAIN 2022
Country/TerritoryUnited States
CityFort Lauderdale
Period24/04/2227/04/22

Bibliographical note

Publisher Copyright:
© 2022 The Author(s).

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