A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Nicolò Accanto; François G.C. Blot; Antonio Lorca Camara; Valeria Zampini; Florence Bui; Christophe Tourain; Noam Badt; Ori Katz; Valentina Emiliani

A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Nicolò Accanto^*
, François G.C. Blot^*
, Antonio Lorca Camara
, Valeria Zampini
, Florence Bui
, Christophe Tourain
, Noam Badt
, Ori Katz
, Valentina Emiliani

^*Corresponding author for this work

The Institute of Applied Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present a new micro-endoscope based on a fiber bundle and a GRIN lens, capable of 2P holographic photostimulation of multiple neurons with single cell precision and fast (100 Hz) 2P calcium imaging on fields of view of 250 μm in freely moving mice.

Original language	English
Title of host publication	Optics and the Brain, BRAIN 2022
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Electronic)	9781957171036
State	Published - 2022
Event	Optics and the Brain, BRAIN 2022 - Fort Lauderdale, United States Duration: 24 Apr 2022 → 27 Apr 2022

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Optics and the Brain, BRAIN 2022
Country/Territory	United States
City	Fort Lauderdale
Period	24/04/22 → 27/04/22

Bibliographical note

Publisher Copyright:
© 2022 The Author(s).

Cite this

Accanto, N., Blot, F. G. C., Camara, A. L., Zampini, V., Bui, F., Tourain, C., Badt, N., Katz, O., & Emiliani, V. (2022). A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals. In Optics and the Brain, BRAIN 2022 Article BW4C.6 (Optics InfoBase Conference Papers). Optica Publishing Group (formerly OSA).

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title = "A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals",

abstract = "We present a new micro-endoscope based on a fiber bundle and a GRIN lens, capable of 2P holographic photostimulation of multiple neurons with single cell precision and fast (100 Hz) 2P calcium imaging on fields of view of 250 μm in freely moving mice.",

author = "Nicol{\`o} Accanto and Blot, \{Fran{\c c}ois G.C.\} and Camara, \{Antonio Lorca\} and Valeria Zampini and Florence Bui and Christophe Tourain and Noam Badt and Ori Katz and Valentina Emiliani",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s).; Optics and the Brain, BRAIN 2022 ; Conference date: 24-04-2022 Through 27-04-2022",

year = "2022",

language = "אנגלית",

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Accanto, N, Blot, FGC, Camara, AL, Zampini, V, Bui, F, Tourain, C, Badt, N, Katz, O & Emiliani, V 2022, A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals. in Optics and the Brain, BRAIN 2022., BW4C.6, Optics InfoBase Conference Papers, Optica Publishing Group (formerly OSA), Optics and the Brain, BRAIN 2022, Fort Lauderdale, United States, 24/04/22.

A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals. / Accanto, Nicolò; Blot, François G.C.; Camara, Antonio Lorca et al.
Optics and the Brain, BRAIN 2022. Optica Publishing Group (formerly OSA), 2022. BW4C.6 (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

AU - Accanto, Nicolò

AU - Blot, François G.C.

AU - Camara, Antonio Lorca

AU - Zampini, Valeria

AU - Bui, Florence

AU - Tourain, Christophe

AU - Badt, Noam

AU - Katz, Ori

AU - Emiliani, Valentina

PY - 2022

Y1 - 2022

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AB - We present a new micro-endoscope based on a fiber bundle and a GRIN lens, capable of 2P holographic photostimulation of multiple neurons with single cell precision and fast (100 Hz) 2P calcium imaging on fields of view of 250 μm in freely moving mice.

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T3 - Optics InfoBase Conference Papers

BT - Optics and the Brain, BRAIN 2022

PB - Optica Publishing Group (formerly OSA)

T2 - Optics and the Brain, BRAIN 2022

Y2 - 24 April 2022 through 27 April 2022

ER -

A flexible two-photon endoscope for fast activity imaging and cell-precise optogenetic photo-stimulation of neurons in freely moving animals

Abstract

Publication series

Conference

Bibliographical note

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