TY - JOUR
T1 - A flexible two-photon fiberscope for fast activity imaging and precise optogenetic photostimulation of neurons in freely moving mice
AU - Accanto, Nicolò
AU - Blot, François G.C.
AU - Lorca-Cámara, Antonio
AU - Zampini, Valeria
AU - Bui, Florence
AU - Tourain, Christophe
AU - Badt, Noam
AU - Katz, Ori
AU - Emiliani, Valentina
N1 - Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2023/1/18
Y1 - 2023/1/18
N2 - We developed a flexible two-photon microendoscope (2P-FENDO) capable of all-optical brain investigation at near cellular resolution in freely moving mice. The system performs fast two-photon (2P) functional imaging and 2P holographic photostimulation of single and multiple cells using axially confined extended spots. Proof-of-principle experiments were performed in freely moving mice co-expressing jGCaMP7s and the opsin ChRmine in the visual or barrel cortex. On a field of view of 250 μm in diameter, we demonstrated functional imaging at a frame rate of up to 50 Hz and precise photostimulation of selected groups of cells. With the capability to simultaneously image and control defined neuronal networks in freely moving animals, 2P-FENDO will enable a precise investigation of neuronal functions in the brain during naturalistic behaviors.
AB - We developed a flexible two-photon microendoscope (2P-FENDO) capable of all-optical brain investigation at near cellular resolution in freely moving mice. The system performs fast two-photon (2P) functional imaging and 2P holographic photostimulation of single and multiple cells using axially confined extended spots. Proof-of-principle experiments were performed in freely moving mice co-expressing jGCaMP7s and the opsin ChRmine in the visual or barrel cortex. On a field of view of 250 μm in diameter, we demonstrated functional imaging at a frame rate of up to 50 Hz and precise photostimulation of selected groups of cells. With the capability to simultaneously image and control defined neuronal networks in freely moving animals, 2P-FENDO will enable a precise investigation of neuronal functions in the brain during naturalistic behaviors.
KW - 2-photon calcium imaging
KW - 2-photon optogenetic photostimulation
KW - GRIN lenses
KW - all-optical neuronal circuit manipulation
KW - computer-generated holography
KW - freely moving mice
KW - microendoscopy
KW - optical fiber bundles
UR - http://www.scopus.com/inward/record.url?scp=85146087849&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2022.10.030
DO - 10.1016/j.neuron.2022.10.030
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C2 - 36395773
AN - SCOPUS:85146087849
SN - 0896-6273
VL - 111
SP - 176-189.e6
JO - Neuron
JF - Neuron
IS - 2
ER -