A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes

R. Harel-Beja, G. Tzuri, V. Portnoy, M. Lotan-Pompan, S. Lev, S. Cohen, N. Dai, L. Yeselson, A. Meir, S. E. Libhaber, E. Avisar, T. Melame, P. van Koert, H. Verbakel, R. Hofstede, H. Volpin, M. Oliver, A. Fougedoire, C. Stalh, J. FauveB. Copes, Z. Fei, J. Giovannoni, N. Ori, E. Lewinsohn, A. Sherman, J. Burger, Y. Tadmor, A. A. Schaffer, N. Katzir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

133 Scopus citations

Abstract

A genetic map of melon enriched for fruit traits was constructed, using a recombinant inbred (RI) population developed from a cross between representatives of the two subspecies of Cucumis melo L.: PI 414723 (subspecies agrestis) and 'Dulce' (subspecies melo). Phenotyping of 99 RI lines was conducted over three seasons in two locations in Israel and the US. The map includes 668 DNA markers (386 SSRs, 76 SNPs, six INDELs and 200 AFLPs), of which 160 were newly developed from fruit ESTs. These ESTs include candidate genes encoding for enzymes of sugar and carotenoid metabolic pathways that were cloned from melon cDNA or identified through mining of the International Cucurbit Genomics Initiative database (http://www. icugi. org/). The map covers 1,222 cM with an average of 2.672 cM between markers. In addition, a skeleton physical map was initiated and 29 melon BACs harboring fruit ESTs were localized to the 12 linkage groups of the map. Altogether, 44 fruit QTLs were identified: 25 confirming QTLs described using other populations and 19 newly described QTLs. The map includes QTLs for fruit sugar content, particularly sucrose, the major sugar affecting sweetness in melon fruit. Six QTLs interacting in an additive manner account for nearly all the difference in sugar content between the two genotypes. Three QTLs for fruit flesh color and carotenoid content were identified. Interestingly, no clear colocalization of QTLs for either sugar or carotenoid content was observed with over 40 genes encoding for enzymes involved in their metabolism. The RI population described here provides a useful resource for further genomics and metabolomics studies in melon, as well as useful markers for breeding for fruit quality.

Original languageAmerican English
Pages (from-to)511-533
Number of pages23
JournalTheoretical And Applied Genetics
Volume121
Issue number3
DOIs
StatePublished - Aug 2010

Bibliographical note

Funding Information:
We are deeply grateful to the staff of the Cancer Research Center and Paediatric Haematology–Oncology at Sheba Medical Center for the Sequenom analysis. We are most thankful to Dr. Ilan Paran for fruitful discussions throughout the project, to Dr. Harry Paris for critically reviewing the manuscript and to Uzi Sa’ar and Fabian Baumkoler for assistance with the field management. We thank the Galilee Technology Center, Migal for the DHPLC analysis.This research was supported by Research Grant Award No. IS-3877-06 from BARD, the United States—Israel Binational Agricultural Research and Development Fund, and in part by Israel Science Foundation Grant No. 386-06. The research was also supported by De Ruiter Seeds, Enza Zaden, Rijk Zwaan, Sakata Seed Corporation, Semilas Fito, Syngenta Seeds and Vilmorin Clause and Cie. Contribution No. 109/2010 of the Agricultural Research Organization, Bet Dagan Israel.

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