A highly efficient method for carnation transformation

Amir Zuker, Asaph Ahroni, Alexander Vainstein

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Transgenic carnation plants were produced using highly regenerative stem segments. The highest efficiency of adventitious shoot regeneration was obtained with segments originating from the stem's top internode. Transformation of cv. White Sim was performed with Agrobacterium tumefaciens carrying the binary vector pCGN7001 containing uidA and nptll genes encoding β-glucuronidase and neomycin-• phosphotransferase, respectively. Prior to inoculation, explants were wounded by microprojectile bombardment. Following selection in the presence of kanamycin, about 63 green shoots per 100 stem segments were recovered. Upon analysis, about 30% of these recovered shoots exhibited strong stable uidA expression throughout the plant. Thus the overall transformation efficiency was ca. 20%. The presence of the uidA and nptll genes in plants stable expressing uidA was confirmed by PCR and Southern blot analyses.

Original languageAmerican English
Title of host publicationIII International Symposium on In Vitro Culture and Horticultural Breeding
PublisherInternational Society for Horticultural Science
Pages373-375
Number of pages3
ISBN (Print)9789066059092
DOIs
StatePublished - 1997

Publication series

NameActa Horticulturae
Volume447
ISSN (Print)0567-7572

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