TY - JOUR
T1 - A highly sensitive solid-phase radioimmunoassay for the assay of Plasmodium falciparum antigens and antibodies
AU - Avraham, H.
AU - Golenser, J.
AU - Gazitt, Y.
AU - Spira, D. T.
AU - Sulitzeanu, D.
PY - 1982/8/27
Y1 - 1982/8/27
N2 - A highly sensitive radioimmunoassay for detection of P. falciparum antibodies and antigens is described. A partially purified P. falciparum antigen preparation is obtained from in vitro cultured parasites enriched after gelatin sedimentation by sonicating the infected red blood cells and precipitating the proteins with 50% saturated ammonium sulfate. The precipitate is dissolved in buffer, ultracentrifuged and used to coat wells of microtiter plates. Anti-P. falciparum antibodies are detected by incubating antiserum dilutions in the coated wells and detecting the bound IgG with radioiodinated staphylococcal protein A. P. falciparum antigens are detected by their ability to inhibit binding of antibodies to the coated wells. Sera of individuals with a history of P. falciparum infection contain antibodies detectable at a dilution of 1 : 75,000. P. falciparum RBC infected in vitro can be detected at levels of parasitemia of the order of 1 parasite or less per 106 RBC.
AB - A highly sensitive radioimmunoassay for detection of P. falciparum antibodies and antigens is described. A partially purified P. falciparum antigen preparation is obtained from in vitro cultured parasites enriched after gelatin sedimentation by sonicating the infected red blood cells and precipitating the proteins with 50% saturated ammonium sulfate. The precipitate is dissolved in buffer, ultracentrifuged and used to coat wells of microtiter plates. Anti-P. falciparum antibodies are detected by incubating antiserum dilutions in the coated wells and detecting the bound IgG with radioiodinated staphylococcal protein A. P. falciparum antigens are detected by their ability to inhibit binding of antibodies to the coated wells. Sera of individuals with a history of P. falciparum infection contain antibodies detectable at a dilution of 1 : 75,000. P. falciparum RBC infected in vitro can be detected at levels of parasitemia of the order of 1 parasite or less per 106 RBC.
KW - malaria antibody assay
KW - malaria antigen assay
KW - P. falciparum
KW - solid-phase radioimmunoassay
UR - http://www.scopus.com/inward/record.url?scp=0019965206&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(82)90240-X
DO - 10.1016/0022-1759(82)90240-X
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C2 - 6752283
AN - SCOPUS:0019965206
SN - 0022-1759
VL - 53
SP - 61
EP - 68
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -