A highly sensitive solid-phase radioimmunoassay for the assay of Plasmodium falciparum antigens and antibodies

H. Avraham*, J. Golenser, Y. Gazitt, D. T. Spira, D. Sulitzeanu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A highly sensitive radioimmunoassay for detection of P. falciparum antibodies and antigens is described. A partially purified P. falciparum antigen preparation is obtained from in vitro cultured parasites enriched after gelatin sedimentation by sonicating the infected red blood cells and precipitating the proteins with 50% saturated ammonium sulfate. The precipitate is dissolved in buffer, ultracentrifuged and used to coat wells of microtiter plates. Anti-P. falciparum antibodies are detected by incubating antiserum dilutions in the coated wells and detecting the bound IgG with radioiodinated staphylococcal protein A. P. falciparum antigens are detected by their ability to inhibit binding of antibodies to the coated wells. Sera of individuals with a history of P. falciparum infection contain antibodies detectable at a dilution of 1 : 75,000. P. falciparum RBC infected in vitro can be detected at levels of parasitemia of the order of 1 parasite or less per 106 RBC.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalJournal of Immunological Methods
Volume53
Issue number1
DOIs
StatePublished - 27 Aug 1982

Keywords

  • malaria antibody assay
  • malaria antigen assay
  • P. falciparum
  • solid-phase radioimmunoassay

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