A Highly Sensitive Square Wave Voltammetry Based Biosensor for Kinase Activity Measurements

Elza Snir; Einav Amit; Assaf Friedler; Shlomo Yitzchaik

doi:10.1002/BIP.22653

A Highly Sensitive Square Wave Voltammetry Based Biosensor for Kinase Activity Measurements

Elza Snir, Einav Amit, Assaf Friedler, Shlomo Yitzchaik^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

An electrochemical biosensor has been developed for ultrasensitive, label-free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole for-mation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 nM of kinase and the dynamic range was 100 nM up to 11 lM. Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 lM, and the dynamic range was 1– 20 lM.

Original language	American English
Pages (from-to)	515-520
Number of pages	6
Journal	Biopolymers
Volume	104
Issue number	5
DOIs	https://doi.org/10.1002/BIP.22653
State	Published - Sep 2015

Bibliographical note

Funding Information:
Correspondence to: Shlomo Yitzchaik, Institute of chemistry and the center for nanoscience and nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel; e-mail: shlomo.yitzchaik@mail.huji.ac.il Contract grant sponsor: SYMONE project (FET-UCOMP thematic area) Contract grant number: FP7 318597 Contract grant sponsor: COST action (HINT) Contract grant number: MP1202 Dedicated to: Prof. Dr. Luis Moroder, Max Planck Institut fu€r Biochemie, Mar-tinsried Germany

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.

Keywords

electrochemistry
kinase biosensor
peptide monolayer

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10.1002/BIP.22653

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title = "A Highly Sensitive Square Wave Voltammetry Based Biosensor for Kinase Activity Measurements",

abstract = "An electrochemical biosensor has been developed for ultrasensitive, label-free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole for-mation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 nM of kinase and the dynamic range was 100 nM up to 11 lM. Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 lM, and the dynamic range was 1– 20 lM.",

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author = "Elza Snir and Einav Amit and Assaf Friedler and Shlomo Yitzchaik",

note = "Funding Information: Correspondence to: Shlomo Yitzchaik, Institute of chemistry and the center for nanoscience and nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel; e-mail: shlomo.yitzchaik@mail.huji.ac.il Contract grant sponsor: SYMONE project (FET-UCOMP thematic area) Contract grant number: FP7 318597 Contract grant sponsor: COST action (HINT) Contract grant number: MP1202 Dedicated to: Prof. Dr. Luis Moroder, Max Planck Institut fu€r Biochemie, Mar-tinsried Germany Publisher Copyright: {\textcopyright} 2015 Wiley Periodicals, Inc.",

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N2 - An electrochemical biosensor has been developed for ultrasensitive, label-free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole for-mation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 nM of kinase and the dynamic range was 100 nM up to 11 lM. Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 lM, and the dynamic range was 1– 20 lM.

AB - An electrochemical biosensor has been developed for ultrasensitive, label-free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole for-mation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 nM of kinase and the dynamic range was 100 nM up to 11 lM. Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 lM, and the dynamic range was 1– 20 lM.

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A Highly Sensitive Square Wave Voltammetry Based Biosensor for Kinase Activity Measurements

Abstract

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