A Highly Sensitive Square Wave Voltammetry Based Biosensor for Kinase Activity Measurements

Elza Snir, Einav Amit, Assaf Friedler, Shlomo Yitzchaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

An electrochemical biosensor has been developed for ultrasensitive, label-free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole for-mation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 nM of kinase and the dynamic range was 100 nM up to 11 lM. Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 lM, and the dynamic range was 1– 20 lM.

Original languageAmerican English
Pages (from-to)515-520
Number of pages6
JournalBiopolymers
Volume104
Issue number5
DOIs
StatePublished - Sep 2015

Bibliographical note

Publisher Copyright:
© 2015 Wiley Periodicals, Inc.

Keywords

  • electrochemistry
  • kinase biosensor
  • peptide monolayer

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