A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein

Eyal Arbely, Ziad Khattari, Guillaume Brotons, Mutaz Akkawi, Tim Salditt, Isaiah T. Arkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

The agent responsible for the recent severe acute respiratory syndrome (SARS) outbreak is a previously unidentified coronavirus. While there is a wealth of epidemiological studies, little if any molecular characterization of SARS coronavirus (SCoV) proteins has been carried out. Here we describe the molecular characterization of SCoV E protein, a critical component of the virus responsible for virion envelope morphogenesis. We conclusively show that SCoV E protein contains an unusually short, palindromic transmembrane helical hairpin around a previously unidentified pseudo-center of symmetry, a structural feature which seems to be unique to SCoV. The hairpin deforms lipid bilayers by way of increasing their curvature, providing for the first time a molecular explanation of E protein's pivotal role in viral budding. The molecular understanding of this critical component of SCoV may represent the beginning of a concerted effort aimed at inhibiting its function, and consequently, viral infectivity.

Original languageAmerican English
Pages (from-to)769-779
Number of pages11
JournalJournal of Molecular Biology
Volume341
Issue number3
DOIs
StatePublished - 13 Aug 2004

Bibliographical note

Funding Information:
This research was supported in part by a grant from the Israel Science Foundation (784/01) to ITA, a grant from the Deutsche Forschungsgemeinschaft grant to ITA, MA and TS and by a grant from Niedersachsen to ITA. ITA wishes to thank Professors A. Panet, I. Ohad and Dr M. Kosloff for helpful discussions.

Keywords

  • BCoV, bovine coronavirus
  • MHV, mouse hepatitis virus
  • SARS coronavirus
  • SARS, severe acute respiratory syndrome
  • SCoV, SARS coronavirus
  • TGEV, transmissible gastroenteritis virus
  • membrane proteins
  • transmembrane helices
  • viral budding

Fingerprint

Dive into the research topics of 'A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein'. Together they form a unique fingerprint.

Cite this