A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA

D. Eilat; R. Asofsky; R. Laskov

A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA

D. Eilat, R. Asofsky, R. Laskov

Research output: Contribution to journal › Article › peer-review

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Abstract

Spleen cells from an unimmunized autoimmune NZB/NZW F₁ female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (10⁹ liter/mole) for the RNA antigen.

Original language	English
Pages (from-to)	766-768
Number of pages	3
Journal	Journal of Immunology
Volume	124
Issue number	2
State	Published - 1980
Externally published	Yes

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abstract = "Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (109 liter/mole) for the RNA antigen.",

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AB - Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (109 liter/mole) for the RNA antigen.

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A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA

Abstract

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