Abstract
Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (109 liter/mole) for the RNA antigen.
| Original language | English |
|---|---|
| Pages (from-to) | 766-768 |
| Number of pages | 3 |
| Journal | Journal of Immunology |
| Volume | 124 |
| Issue number | 2 |
| State | Published - 1980 |
| Externally published | Yes |
