A Massively Parallel Reporter Assay of 3′ UTR Sequences Identifies In Vivo Rules for mRNA Degradation

Michal Rabani*, Lindsey Pieper, Guo Liang Chew, Alexander F. Schier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

The stability of mRNAs is regulated by signals within their sequences, but a systematic and predictive understanding of the underlying sequence rules remains elusive. Here we introduce UTR-seq, a combination of massively parallel reporter assays and regression models, to survey the dynamics of tens of thousands of 3′ UTR sequences during early zebrafish embryogenesis. UTR-seq revealed two temporal degradation programs: a maternally encoded early-onset program and a late-onset program that accelerated degradation after zygotic genome activation. Three signals regulated early-onset rates: stabilizing poly-U and UUAG sequences and destabilizing GC-rich signals. Three signals explained late-onset degradation: miR-430 seeds, AU-rich sequences, and Pumilio recognition sites. Sequence-based regression models translated 3′ UTRs into their unique decay patterns and predicted the in vivo effect of sequence signals on mRNA stability. Their application led to the successful design of artificial 3′ UTRs that conferred specific mRNA dynamics. UTR-seq provides a general strategy to uncover the rules of RNA cis regulation. The sequences of mRNAs affect their stability. Rabani et al. introduce UTR-seq to uncover the rules that translate mRNA sequences into decay patterns. They survey the decay of tens of thousands of mRNAs, identify sequences that regulate mRNA degradation during early zebrafish embryogenesis, and establish sequence-based models that predict mRNA decay.

Original languageAmerican English
Pages (from-to)1083-1094.e5
JournalMolecular Cell
Volume68
Issue number6
DOIs
StatePublished - 2017
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2017 Elsevier Inc.

Keywords

  • 3′
  • RNA degradation
  • RNA stability regulation
  • UTR
  • massively parallel reporter assay
  • maternal to zygotic transition

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