TY - JOUR
T1 - A method for measuring anion transfer across red cell membranes by continuous monitoring of fluorescence
AU - Eidelman, O.
AU - Cabantchik, Z. I.
PY - 1980
Y1 - 1980
N2 - A fluorometric method for continuous monitoring of anion transport across red blood cell membranes was developed. The method was based on the use of a fluorescent substrate, N-(2-aminoethylsulfonate)-7-nitrobenz-2-oxa-3-diazole) (NBD-taurine), whose intracellular fluorescence was quenched by hemoglobin. Cells loaded with NBD-taurine, free of extracellular probe, were suspended in various media, and the efflux of probe was recorded as a time-dependent increase in fluorescence. The method was highly reproducible and was sensitive down to 103 cells as well as to transport rates in the range 10–30 s. A single fluorescence trace allowed determination of the Michaelis-Menten parameters of NBD-taurine efflux.
AB - A fluorometric method for continuous monitoring of anion transport across red blood cell membranes was developed. The method was based on the use of a fluorescent substrate, N-(2-aminoethylsulfonate)-7-nitrobenz-2-oxa-3-diazole) (NBD-taurine), whose intracellular fluorescence was quenched by hemoglobin. Cells loaded with NBD-taurine, free of extracellular probe, were suspended in various media, and the efflux of probe was recorded as a time-dependent increase in fluorescence. The method was highly reproducible and was sensitive down to 103 cells as well as to transport rates in the range 10–30 s. A single fluorescence trace allowed determination of the Michaelis-Menten parameters of NBD-taurine efflux.
UR - http://www.scopus.com/inward/record.url?scp=0019133549&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(80)90529-1
DO - 10.1016/0003-2697(80)90529-1
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C2 - 6255819
AN - SCOPUS:0019133549
SN - 0003-2697
VL - 106
SP - 335
EP - 341
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -