A method for measuring picosecond phenomena in photolabile species: the emission lifetime of bacteriorhodopsin

M. D. Hirsch*, M. A. Marcus, A. Lewis, H. Mahr, N. Frigo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

We have measured the emission lifetime of bacteriorhodopsin at physiological temperatures to be 15 +/- 3 ps using a technique which employs a mode-locked dye laser, a sum frequency light gate, and a continuous flow system. We observe no concentration dependence of the lifetime over the range of 1.1 X 10(-4) M to 1.0 X 10(-5) M. We conclude that the emission which we observe comes from bacteriorhodopsin and not one of its photochemically produced intermediates, and that the emission cannot originate from the state into which light is absorbed.

Original languageEnglish
Pages (from-to)1399-1409
Number of pages11
JournalBiophysical Journal
Volume16
Issue number12
DOIs
StatePublished - 1976

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