A method for measuring sulfide toxicity in the nematode Caenorhabditis elegans

Leonid Livshits, Einav Gross*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Cysteine catabolism by gut microbiota produces high levels of sulfide. Excessive sulfide can interfere with colon function, and therefore may be involved in the etiology and risk of relapse of ulcerative colitis, an inflammatory bowel disease affecting millions of people worldwide. Therefore, it is crucial to understand how cells/animals regulate the detoxification of sulfide generated by bacterial cysteine catabolism in the gut. Here we describe a simple and cost-effective way to explore the mechanism of sulfide toxicity in the nematode Caenorhabditis elegans (C. elegans). • A rapid cost-effective method to quantify and study sulfide tolerance in C. elegans and other free-living nematodes.• A cost effective method to measure the concentration of sulfide in the inverted plate assay.

Original languageAmerican English
Pages (from-to)250-255
Number of pages6
JournalMethodsX
Volume4
DOIs
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017 The Author(s)

Keywords

  • C. elegans
  • Cysteine catabolism
  • Methylene blue
  • Sulfide
  • egl-9

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