A method for the measurement of chloroquine uptake in erythrocytes

A. Yayon*, H. Ginsburg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The properties of adsorption of chloroquine to glass surfaces have been used to develop an accurate method for the determination of initial rates of uptake of this drug into human erythrocytes. The method involves a rapid passage of erythrocytes suspended in chloroquine solution through a minicolumn filled with controlled-pore glass beads. The column retains more than 99% of the extracellular chloroquine and a far smaller percentage of cells. Sampling times for uptake measurements were down to 4 s. A procedure is described for the regeneration of the column for further use.

Original languageEnglish
Pages (from-to)332-336
Number of pages5
JournalAnalytical Biochemistry
Volume107
Issue number2
DOIs
StatePublished - 1980

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