A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells

A. Shamay*, A. Gertler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A method for culturing bovine undifferentiated mammary epithelial cells embedded in collagen gels is described. Cell growth was quantitated by incorporation of tritiated thymidine. Maximal rate of incorporation was achieved in media supplemented with sera, however considerable growth was observed in serum-free medium supplemented with insulin, transferrin and selenium. The growth promoting effect of insulin was maximally expressed only at high nonphysiological concentrations.

Original languageEnglish
Pages (from-to)923-929
Number of pages7
JournalCell Biology International Reports
Volume10
Issue number12
DOIs
StatePublished - Dec 1986

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