A modified procedure for the large scale preparation of tRNA from E. coli

S. Bauer*, Y. Milner, E. Ziv, J. Shiloach, N. De Groot, Y. Lapidot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A procedure for the preparation of about 50 g batches of tRNA from 25 kg E. coli W is described. The method involves phenolic extraction of the cells, batch absorption of the tRNA on DEAE‐cellulose, washing the DEAE‐cellulose and packing it into a column, elution of the tRNA from the column and precipitation of the tRNA with ethanol. The method is less time and labor consuming than the methods described in the literature and can be carried out with relatively simple equipment.

Original languageEnglish
Pages (from-to)1081-1088
Number of pages8
JournalBiotechnology and Bioengineering
Volume15
Issue number6
DOIs
StatePublished - Nov 1973

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