TY - JOUR
T1 - A modified procedure for the large scale preparation of tRNA from E. coli
AU - Bauer, S.
AU - Milner, Y.
AU - Ziv, E.
AU - Shiloach, J.
AU - De Groot, N.
AU - Lapidot, Y.
PY - 1973/11
Y1 - 1973/11
N2 - A procedure for the preparation of about 50 g batches of tRNA from 25 kg E. coli W is described. The method involves phenolic extraction of the cells, batch absorption of the tRNA on DEAE‐cellulose, washing the DEAE‐cellulose and packing it into a column, elution of the tRNA from the column and precipitation of the tRNA with ethanol. The method is less time and labor consuming than the methods described in the literature and can be carried out with relatively simple equipment.
AB - A procedure for the preparation of about 50 g batches of tRNA from 25 kg E. coli W is described. The method involves phenolic extraction of the cells, batch absorption of the tRNA on DEAE‐cellulose, washing the DEAE‐cellulose and packing it into a column, elution of the tRNA from the column and precipitation of the tRNA with ethanol. The method is less time and labor consuming than the methods described in the literature and can be carried out with relatively simple equipment.
UR - http://www.scopus.com/inward/record.url?scp=0015759294&partnerID=8YFLogxK
U2 - 10.1002/bit.260150607
DO - 10.1002/bit.260150607
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C2 - 4587923
AN - SCOPUS:0015759294
SN - 0006-3592
VL - 15
SP - 1081
EP - 1088
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 6
ER -