A modified procedure for the preparation of di-and triribonucleotides from pancreatic ribonuclease digest of RNA

S. Bauer; M. D. White; Y. Lapidot

doi:10.1093/nar/2.12.2355

A modified procedure for the preparation of di-and triribonucleotides from pancreatic ribonuclease digest of RNA

S. Bauer^*, M. D. White, Y. Lapidot

^*Corresponding author for this work

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Abstract

A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH₄ HCO₃.

Original language	English
Pages (from-to)	2355-2364
Number of pages	10
Journal	Nucleic Acids Research
Volume	2
Issue number	12
DOIs	https://doi.org/10.1093/nar/2.12.2355
State	Published - Dec 1975

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title = "A modified procedure for the preparation of di-and triribonucleotides from pancreatic ribonuclease digest of RNA",

abstract = "A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4 HCO3.",

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N2 - A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4 HCO3.

AB - A novel procedure for the separation of oligonucleotides from pancreatic RNase-digest of RNA is described. The method involves a group-separation of uracil-containing and of cytosine-containing nucleotides on Dowex 50W. The obtained groups are further separated on DEAE-Sephadex A-25 by a linear gradient of NH4 HCO3.

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A modified procedure for the preparation of di-and triribonucleotides from pancreatic ribonuclease digest of RNA

Abstract

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