A molecular genetic linkage map of mouse chromosome 2

Linda D. Siracusa, Colleen M. Silan, Monica J. Justice, John A. Mercer, Asne R. Bauskin, Yinon Ben-Neriah, Denis Duboule, Nicholas D. Hastie, Neal G. Copeland, Nancy A. Jenkins*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

Interspecific backcross mice were used to create a molecular genetic linkage map of chromosome 2. Genomic DNAs from N2 progeny were subjected to Southern blot analysis using molecular probes that identified the Abl, Acra, Ass, C5, Cas-1, Fshb, Geg, Hox-5.1, Jgf-1, Kras-3, Ltk, Pax-1, Prn-p, and Spna-2 loci; these loci were added to the 11 loci previously mapped to the distal region of chromosome 2 in the same interspecific backcross to generate a composite multilocus linkage map. Several loci mapped near, and may be the same as, known mutations. Comparisons between the mouse and the human genomes indicate that mouse chromosome 2 contains regions homologous to at least six human chromosomes. Mouse models for human diseases are discussed.

Original languageAmerican English
Pages (from-to)491-504
Number of pages14
JournalGenomics
Volume6
Issue number3
DOIs
StatePublished - Mar 1990

Bibliographical note

Funding Information:
We thank P. C. Watkins, B. F. Tack, G. F. Saunders, G. Radice, J. P. Merlie, B. A. Mock, T. L. Leto, J. Lee, E. M. Either, F. Cos-tantini, and G. A. Carlson for generously sharing their resources with us. We thank M. F. Lyon and D. P. Doolittle for valuable scientific discussions and for granting us permission to publish their maps of mouse chromosome 2. We thank A. S. Perkins, I. J. Jackson, A. M. Buchberg, and W. A. Bickmore for critically reviewing the manuscript. We thank A. M. Buchberg for help in constructing the figures. We thank S. Yang, D. A. Swing, A. Seawright, B. Eagleson, J. Diem, W. Chan, and M. Bodamer for excellent technical assistance. The research was supported in part by the National Cancer Institute, DHHS, under Contract NOl-CO-74101 with BRI. NCI-FCRF is fully accredited by the American Association for Accreditation of Laboratory Animal Care. L.D.S. is the recipient of a National Research Service Award, Postdoctoral Fellowship GM12721-01. J.A.M. is a Leukemia Society of America Fellow.

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