A new hyperrecombinogenic mutant of Nicotiana tabacum

Vera Gorbunova, Naomi Avivi-Ragolski, Gil Shalev, Igor Kovalchuk, Shahal Abbo, Barbara Hohn, Avraham A. Levy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We have isolated a hyperrecombinogenic Nicotiana tabacum mutant. The mutation, Hyrec, is dominant and segregates in a Mendelian fashion. In the mutant, the level of mitotic recombination between homologous chromosomes is increased by more than three orders of magnitude. Recombination between extrachromosomal substrates is increased six- to ninefold, and intrachromosomal recombination is not affected. Hyrec plants were found to perform non-homologous end joining as efficiently as the wild type, ruling out the possibility that the increase in homologous recombination is due to a defect in end joining. In addition, Hyrec plants show significant resistance to gamma-irradiation, whereas UV resistance is not different from the wild type. This suggests that homologous recombination can be strongly up-regulated in plants. Moreover, Hyrec constitutes a novel type of mutation: no similar mutant was reported in plants and hyperrecombinogenic mutants from other organisms usually show sensitivity to DNA damaging agents. We discuss the insight that this mutant provides into understanding the mechanisms of recombination plus the potential application for gene targeting in plants.

Original languageAmerican English
Pages (from-to)601-611
Number of pages11
JournalPlant Journal
Volume24
Issue number5
DOIs
StatePublished - 2000

Keywords

  • Gene targeting
  • Hyperrecombinogenic mutants
  • Nicotiana tabacum
  • Recombination

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