A new method for reconstitution of highly fusogenic sendai virus envelopes

Alexander Vainstein; Maty Hershkovitz; Shoshana Israel; Sophia Rabin; Abraham Loyter

doi:10.1016/0005-2736(84)90081-6

A new method for reconstitution of highly fusogenic sendai virus envelopes

Alexander Vainstein, Maty Hershkovitz, Shoshana Israel, Sophia Rabin, Abraham Loyter^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.

Original language	English
Pages (from-to)	181-188
Number of pages	8
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	773
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(84)90081-6
State	Published - 27 Jun 1984

Bibliographical note

Funding Information:
This work was supported by a grant, No. 2481/81, from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel.

Keywords

(Sendai virus)
Membrane fusion
Membrane reconstitution
Virus envelope

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10.1016/0005-2736(84)90081-6

Cite this

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abstract = "A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.",

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author = "Alexander Vainstein and Maty Hershkovitz and Shoshana Israel and Sophia Rabin and Abraham Loyter",

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AU - Vainstein, Alexander

AU - Hershkovitz, Maty

AU - Israel, Shoshana

AU - Rabin, Sophia

AU - Loyter, Abraham

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AB - A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.

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A new method for reconstitution of highly fusogenic sendai virus envelopes

Abstract

Bibliographical note

Keywords

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