A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.
Bibliographical noteFunding Information:
This work was supported by a grant, No. 2481/81, from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel.
- (Sendai virus)
- Membrane fusion
- Membrane reconstitution
- Virus envelope