A new method for reconstitution of highly fusogenic sendai virus envelopes

Alexander Vainstein, Maty Hershkovitz, Shoshana Israel, Sophia Rabin, Abraham Loyter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.

Original languageAmerican English
Pages (from-to)181-188
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume773
Issue number2
DOIs
StatePublished - 27 Jun 1984

Bibliographical note

Funding Information:
This work was supported by a grant, No. 2481/81, from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel.

Keywords

  • (Sendai virus)
  • Membrane fusion
  • Membrane reconstitution
  • Virus envelope

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