A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro

Anna Melkov, Raju Baskar, Yehonatan Alcalay, Uri Abdu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Intrinsic cell microtubule (MT) polarity, together with molecular motors and adaptor proteins, determines mitochondrial polarized targeting and MT-dependent transport. In polarized cells, such as neurons, mitochondrialmobility and transport require the regulation of kinesin and dynein by two adaptor proteins,Milton and Miro. Recently, we found that dynein heavy chain 64C (Dhc64C) is the primary motor protein for both anterograde and retrograde transport of mitochondria in the Drosophila bristle. In this study, we show that a molecular lesion in the Dhc64C allele that reduced bristle mitochondrial velocity generated a variant that acts as a ‘slow’ dynein in an MT-gliding assay, indicating that dynein directly regulates mitochondrial transport. We also showed that in milton-RNAi flies, mitochondrial flux into the bristle shaft, but not velocity, was significantly reduced. Surprisingly, mitochondria retrograde flux, but not net velocity, was significantly decreased in miro-RNAi flies.We thus reveal a new mode of mitochondrial sorting in polarized cell growth, whereby bi-directional mitochondrial transport undertaken exclusively by dynein is regulated by Milton in the anterograde direction and by a Miro-dependent switch to the retrograde direction.

Original languageAmerican English
Pages (from-to)4203-4213
Number of pages11
JournalDevelopment (Cambridge)
Volume143
Issue number22
DOIs
StatePublished - 15 Nov 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016. Published by The Company of Biologists Ltd.

Keywords

  • Bristle
  • Drosophila
  • Dynein
  • Milton
  • Miro
  • Mitochondria

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