TY - JOUR
T1 - A new mode of probiotic therapy
T2 - Specific targeting
AU - Amit-Romach, Einat
AU - Uni, Zehava
AU - Friedman, Michael
AU - Aizenberg, Itzhak
AU - Berkovich, Zippi
AU - Reifen, Ram
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Luminal bacteria are the most probable inducers of inflammatory bowel disease (IBD). The intestinal microbiota can be modified by probiotics, which reduce symptoms in IBD, via stimulation of the intestinal immune system. We aimed to evaluate the effects of oral and rectal administration of probiotics, on the morphology, gene expression, and microbial ecology of the colon, in a rat trinitrobenzenesulfonic acid (TNBS) model. Colitis was induced with simultaneous rectal or oral administration of probiotics. Both routes of introduction significantly increased the relative amounts of Lactobacillus spp. Rectal introduction almost eradicated damage to the tissue, while enteral introduction only improved it. Only rectal probiotics significantly decreased myeloperoxidase activity, and altered mucin and Toll-like receptor mRNA expression in the colon, to values close to control. We suggest that targeted introduction should be considered not only for probiotics but also for nutrients such as amino acids and vitamins into the colon and intestine.
AB - Luminal bacteria are the most probable inducers of inflammatory bowel disease (IBD). The intestinal microbiota can be modified by probiotics, which reduce symptoms in IBD, via stimulation of the intestinal immune system. We aimed to evaluate the effects of oral and rectal administration of probiotics, on the morphology, gene expression, and microbial ecology of the colon, in a rat trinitrobenzenesulfonic acid (TNBS) model. Colitis was induced with simultaneous rectal or oral administration of probiotics. Both routes of introduction significantly increased the relative amounts of Lactobacillus spp. Rectal introduction almost eradicated damage to the tissue, while enteral introduction only improved it. Only rectal probiotics significantly decreased myeloperoxidase activity, and altered mucin and Toll-like receptor mRNA expression in the colon, to values close to control. We suggest that targeted introduction should be considered not only for probiotics but also for nutrients such as amino acids and vitamins into the colon and intestine.
KW - Colitis
KW - Probiotics
KW - Targeting
KW - Therapy
UR - http://www.scopus.com/inward/record.url?scp=84937559498&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2015.04.029
DO - 10.1016/j.jff.2015.04.029
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AN - SCOPUS:84937559498
SN - 1756-4646
VL - 16
SP - 386
EP - 392
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -