TY - JOUR
T1 - A Note on the Detection of Phytopathogenic Bacteria within the Leaf by a Differential Staining Procedure
AU - BASHAN, Y.
AU - KRITZMAN, G.
AU - SHARON, EDNA
AU - OKON, Y.
AU - HENIS, Y.
PY - 1981/4
Y1 - 1981/4
N2 - A simple differential staining procedure for demonstrating infection within the leaf tissue by Pseudomonas tomato, Ps. lachrymans and Xanthomonas vesicatoria has been developed. It is based on (1) clearing of plant tissue with a mixture of glycerol, lactic acid, phenol and water; (2) treating the leaf tissue with boiling KOH; and (3) staining with aniline blue‐chloralhydrate. When observed under a light microscope, the bacteria appear dark blue, whereas the leaf tissue appears transparent and colourless.
AB - A simple differential staining procedure for demonstrating infection within the leaf tissue by Pseudomonas tomato, Ps. lachrymans and Xanthomonas vesicatoria has been developed. It is based on (1) clearing of plant tissue with a mixture of glycerol, lactic acid, phenol and water; (2) treating the leaf tissue with boiling KOH; and (3) staining with aniline blue‐chloralhydrate. When observed under a light microscope, the bacteria appear dark blue, whereas the leaf tissue appears transparent and colourless.
UR - http://www.scopus.com/inward/record.url?scp=84980204598&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.1981.tb00896.x
DO - 10.1111/j.1365-2672.1981.tb00896.x
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AN - SCOPUS:84980204598
SN - 0021-8847
VL - 50
SP - 315
EP - 317
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
IS - 2
ER -