A Note on the Detection of Phytopathogenic Bacteria within the Leaf by a Differential Staining Procedure

Y. BASHAN; G. KRITZMAN; EDNA SHARON; Y. OKON; Y. HENIS

doi:10.1111/j.1365-2672.1981.tb00896.x

A Note on the Detection of Phytopathogenic Bacteria within the Leaf by a Differential Staining Procedure

Y. BASHAN^*
, G. KRITZMAN
, EDNA SHARON
, Y. OKON
, Y. HENIS

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A simple differential staining procedure for demonstrating infection within the leaf tissue by Pseudomonas tomato, Ps. lachrymans and Xanthomonas vesicatoria has been developed. It is based on (1) clearing of plant tissue with a mixture of glycerol, lactic acid, phenol and water; (2) treating the leaf tissue with boiling KOH; and (3) staining with aniline blue‐chloralhydrate. When observed under a light microscope, the bacteria appear dark blue, whereas the leaf tissue appears transparent and colourless.

Original language	English
Pages (from-to)	315-317
Number of pages	3
Journal	Journal of Applied Bacteriology
Volume	50
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2672.1981.tb00896.x
State	Published - Apr 1981

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title = "A Note on the Detection of Phytopathogenic Bacteria within the Leaf by a Differential Staining Procedure",

abstract = "A simple differential staining procedure for demonstrating infection within the leaf tissue by Pseudomonas tomato, Ps. lachrymans and Xanthomonas vesicatoria has been developed. It is based on (1) clearing of plant tissue with a mixture of glycerol, lactic acid, phenol and water; (2) treating the leaf tissue with boiling KOH; and (3) staining with aniline blue‐chloralhydrate. When observed under a light microscope, the bacteria appear dark blue, whereas the leaf tissue appears transparent and colourless.",

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A Note on the Detection of Phytopathogenic Bacteria within the Leaf by a Differential Staining Procedure

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