A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene

Koyu Hon-Nami, Sadao Ueno, Hideki Endo, Hiroyuki Nishimura, Takashi Igarashi, Lior David, Shintaro Iwashita

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The ruminant-specific p97bcnt gene (bcnt p97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcnt p97 paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcnt p97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcnt p97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.

Original languageAmerican English
Pages (from-to)283-290
Number of pages8
JournalGene
Volume340
Issue number2
DOIs
StatePublished - 13 Oct 2004
Externally publishedYes

Bibliographical note

Funding Information:
We thank Drs. Hidetoshi B. Tamate, Hiroshi Nakashima, and anonymous referees for valuable comments on the manuscript, and Dr. Showbu Sato and Yumiko Sano and Ms. Ako Tokumasu for technical advice. We are grateful for the encouragement of the former President Yoshitaka Nagai, Dr. Katsuhiro Fukuta, and Dr. Mitsuko Masui. The work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology (#13575027).

Keywords

  • GA-repeat
  • Gene duplication
  • Gene fixation
  • Ruminantia

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