A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene

Koyu Hon-Nami; Sadao Ueno; Hideki Endo; Hiroyuki Nishimura; Takashi Igarashi; Lior David; Shintaro Iwashita

doi:10.1016/j.gene.2004.07.016

A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene

Koyu Hon-Nami, Sadao Ueno, Hideki Endo, Hiroyuki Nishimura, Takashi Igarashi, Lior David, Shintaro Iwashita

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The ruminant-specific p97bcnt gene (bcnt ^p97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcnt ^p97 paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcnt ^p97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcnt ^p97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.

Original language	English
Pages (from-to)	283-290
Number of pages	8
Journal	Gene
Volume	340
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2004.07.016
State	Published - 13 Oct 2004
Externally published	Yes

Bibliographical note

Funding Information:
We thank Drs. Hidetoshi B. Tamate, Hiroshi Nakashima, and anonymous referees for valuable comments on the manuscript, and Dr. Showbu Sato and Yumiko Sano and Ms. Ako Tokumasu for technical advice. We are grateful for the encouragement of the former President Yoshitaka Nagai, Dr. Katsuhiro Fukuta, and Dr. Mitsuko Masui. The work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology (#13575027).

Keywords

GA-repeat
Gene duplication
Gene fixation
Ruminantia

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10.1016/j.gene.2004.07.016

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title = "A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene",

abstract = "The ruminant-specific p97bcnt gene (bcnt p97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcnt p97 paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcnt p97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcnt p97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.",

keywords = "GA-repeat, Gene duplication, Gene fixation, Ruminantia",

author = "Koyu Hon-Nami and Sadao Ueno and Hideki Endo and Hiroyuki Nishimura and Takashi Igarashi and Lior David and Shintaro Iwashita",

note = "Funding Information: We thank Drs. Hidetoshi B. Tamate, Hiroshi Nakashima, and anonymous referees for valuable comments on the manuscript, and Dr. Showbu Sato and Yumiko Sano and Ms. Ako Tokumasu for technical advice. We are grateful for the encouragement of the former President Yoshitaka Nagai, Dr. Katsuhiro Fukuta, and Dr. Mitsuko Masui. The work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology (#13575027).",

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doi = "10.1016/j.gene.2004.07.016",

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AU - Hon-Nami, Koyu

AU - Ueno, Sadao

AU - Endo, Hideki

AU - Nishimura, Hiroyuki

AU - Igarashi, Takashi

AU - David, Lior

AU - Iwashita, Shintaro

N1 - Funding Information: We thank Drs. Hidetoshi B. Tamate, Hiroshi Nakashima, and anonymous referees for valuable comments on the manuscript, and Dr. Showbu Sato and Yumiko Sano and Ms. Ako Tokumasu for technical advice. We are grateful for the encouragement of the former President Yoshitaka Nagai, Dr. Katsuhiro Fukuta, and Dr. Mitsuko Masui. The work was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology (#13575027).

PY - 2004/10/13

Y1 - 2004/10/13

N2 - The ruminant-specific p97bcnt gene (bcnt p97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcnt p97 paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcnt p97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcnt p97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.

AB - The ruminant-specific p97bcnt gene (bcnt p97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcnt p97 paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcnt p97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcnt p97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.

KW - GA-repeat

KW - Gene duplication

KW - Gene fixation

KW - Ruminantia

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A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene

Abstract

Bibliographical note

Keywords

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