TY - JOUR
T1 - A novel method for quantitation of favism-inducing agents in legumes
AU - Chevion, Mordechai
AU - Navok, Tikva
PY - 1983/1
Y1 - 1983/1
N2 - A new method for the quantitation of the favism-inducing agents in legumes is described. The procedure involves differential extraction of the glucosides vicine and convicine with acetic acid (25%), followed by an enzymatic hydrolysis by β-glucosidase under anaerobic conditions. Each of the aglycone moieties, isouramil and divicine, anaerobically reduces two molecules of o-ferriphenanthroline to o-ferrophenanthroline. This reaction is readily followed spectrophotometrically at 515 nm. Using this procedure, it was found that in various strains of Vicia faba, the level of these two glucosides comprises ∼0.5% of the wet weight of the seeds. In contrast, these glucosides could not be detected in either green peas or chick peas.
AB - A new method for the quantitation of the favism-inducing agents in legumes is described. The procedure involves differential extraction of the glucosides vicine and convicine with acetic acid (25%), followed by an enzymatic hydrolysis by β-glucosidase under anaerobic conditions. Each of the aglycone moieties, isouramil and divicine, anaerobically reduces two molecules of o-ferriphenanthroline to o-ferrophenanthroline. This reaction is readily followed spectrophotometrically at 515 nm. Using this procedure, it was found that in various strains of Vicia faba, the level of these two glucosides comprises ∼0.5% of the wet weight of the seeds. In contrast, these glucosides could not be detected in either green peas or chick peas.
UR - http://www.scopus.com/inward/record.url?scp=0020655483&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(83)90355-X
DO - 10.1016/0003-2697(83)90355-X
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 6846790
AN - SCOPUS:0020655483
SN - 0003-2697
VL - 128
SP - 152
EP - 158
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -