A novel phenotypic method to screen for plasmid-mediated colistin resistance among enterobacteriales

Drew T. Bell; Yehudit Bergman; Abida Q. Kazmi; Shawna Lewis; Pranita D. Tamma; Patricia J. Simner

doi:10.1128/JCM.00040-19

A novel phenotypic method to screen for plasmid-mediated colistin resistance among enterobacteriales

Drew T. Bell, Yehudit Bergman, Abida Q. Kazmi, Shawna Lewis, Pranita D. Tamma, Patricia J. Simner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test should be further evaluated to confirm the presence of mcr genes.

Original language	English
Article number	e00040-19
Journal	Journal of Clinical Microbiology
Volume	57
Issue number	5
DOIs	https://doi.org/10.1128/JCM.00040-19
State	Published - May 2019
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2019 American Society for Microbiology.

Keywords

Colistin
Mcr
Phenotypic method
Plasmid-mediated colistin resistance

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title = "A novel phenotypic method to screen for plasmid-mediated colistin resistance among enterobacteriales",

abstract = "Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test should be further evaluated to confirm the presence of mcr genes.",

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doi = "10.1128/JCM.00040-19",

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AU - Kazmi, Abida Q.

AU - Lewis, Shawna

AU - Tamma, Pranita D.

AU - Simner, Patricia J.

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AB - Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test should be further evaluated to confirm the presence of mcr genes.

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A novel phenotypic method to screen for plasmid-mediated colistin resistance among enterobacteriales

Abstract

Bibliographical note

Keywords

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